Porcine APLN (Apelin) ELISA Kit | APLN elisa kit

Porcine APLN (Apelin) ELISA Kit

Cat. Num. AAA7606734

• Gene Names: APLN; APEL; XNPEP2
• Reactivity: Porcine
• Synonyms: APLN (Apelin); Porcine APLN (Apelin) ELISA Kit; APLN elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANXA1. No significant cross-reactivity or interference between ANXA1 and analogues was observed.
• Sequence Length: 77

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANXA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANXA1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APLN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ANXA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ANXA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANXA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANXA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6708146
• NCBI GeneID: 8862
• Molecular Weight: 8,569 Da
• NCBI Official Full Name: apelin
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: APLN
• NCBI Official Synonym Symbols: APEL; XNPEP2
• NCBI Protein Information: apelin
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: APLN
• UniProt Synonym Gene Names: APEL

NCBI Description

This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016]

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; receptor binding

Biological Process: immune response; lactation