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Western Blot (WB)
(Western Blot analysis of Hela cells using FoxO1 Polyclonal Antibody at dilution of 1:500.)
Forkhead Signaling Antibody Kit
Forkhead Signaling Antibody Sampler Kit
Applications
Western Blot
Synonyms
Forkhead Signaling; Forkhead Signaling Antibody Sampler Kit; Forkhead Signaling antibody kit
Applicable Applications for Forkhead Signaling antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Hela cells using FoxO1 Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of Hela cells using FoxO1 Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-FoxO1 (Ser256) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-FoxO1 (Ser256) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 3T3 cells with Phospho-FoxO1 (Ser319) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of 3T3 cells with Phospho-FoxO1 (Ser319) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of 3T3 cells with Phospho-FoxO1/3 (Ser322/S325) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of 3T3 cells with Phospho-FoxO1/3 (Ser322/S325) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of Jurkat cells using FoxO3A Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Jurkat cells using FoxO3A Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-FoxO3A (Ser253) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-FoxO3A (Ser253) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of COS7 cells with FoxO4 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COS7 cells with FoxO4 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with Phospho-FoxO4 (Ser197) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with Phospho-FoxO4 (Ser197) Polyclonal Antibody)
Related Product Information for Forkhead Signaling antibody kit
Principle of the Assay: This sampler kit provides an economical means to investigate Forkhead signaling. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Background/Introduction: The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias. Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt. Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-beta-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K. Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity. Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1).
Background/Introduction: The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias. Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt. Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-beta-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K. Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity. Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1).
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Product Notes
The Forkhead Signaling (Catalog #AAA2550033) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Forkhead Signaling can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Forkhead Signaling for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Forkhead Signaling, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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