Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FOXO3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Rabbit FoxO3a Polyclonal Antibody | anti-FOXO3 antibody
FoxO3a Rabbit pab Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry
Purity
Affinity purification
Synonyms
FoxO3a; Polyclonal Antibody; FoxO3a Rabbit pab Antibody; FOXO3; FoxO3a ; AF6q21; DKFZp781A0677; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; MGC12739; MGC31925; anti-FOXO3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
673
Applicable Applications for anti-FOXO3 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Western Blot (WB): 1/500-1000
Immunocytochemistry (ICC): 1/20-50
Immunocytochemistry (ICC): 1/20-50
Immunogen
A synthetic -peptide of human FoxO3a
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FOXO3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FOXO3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using FOXO3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using FOXO3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver injury using FOXO3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver injury using FOXO3 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-FOXO3 antibody
Rabbit polyclonal to FoxO3a
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-beta-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). In Fox03a, the three sites phosphorylated by Akt mentioned above are Thr32, Ser253 and Ser315. Fox03a associates with 14-3-3 proteins upon phosphorylation by Akt and is retained in the cytoplasm. In the absence of survival factors, Fox03a is dephosphorylated, translocates to the nucleus and triggers cell death by a Fas ligand-dependent mechanism (7).
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-beta-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). In Fox03a, the three sites phosphorylated by Akt mentioned above are Thr32, Ser253 and Ser315. Fox03a associates with 14-3-3 proteins upon phosphorylation by Akt and is retained in the cytoplasm. In the absence of survival factors, Fox03a is dephosphorylated, translocates to the nucleus and triggers cell death by a Fas ligand-dependent mechanism (7).
References
1. Anderson, M.J. et al. (1998) Genomics 47, 187-199. 2. Galili, N. et al. (1993) Nat. Genet. 5, 230-235. 3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202. 4. Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982-15985. 5. Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183. 6. Guo, S. et al. (1999) J. Biol. Chem. 274, 17184-17192. 7. Seoane, J. et al. (2004) Cell 117, 211-223. 8. Arden, K.C. (2004) Mol. Cell 14, 416-418. 9. Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW 44kDa
Calculated MW 33kDa/44kDa
Calculated MW 33kDa/44kDa
NCBI Official Full Name
forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
NCBI Protein Information
forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
UniProt Protein Name
Forkhead box protein O3
Protein Family
UniProt Gene Name
FOXO3
UniProt Synonym Gene Names
FKHRL1; FOXO3A
UniProt Entry Name
FOXO3_HUMAN
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Product Notes
The FOXO3 foxo3 (Catalog #AAA125231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FoxO3a Rabbit pab Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FoxO3a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Western Blot (WB): 1/500-1000 Immunocytochemistry (ICC): 1/20-50. Researchers should empirically determine the suitability of the FOXO3 foxo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FoxO3a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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