NF-kappaB p65 Antibody Kit

NF-kappaB p65 Antibody Sampler Kit

Cat. Num. AAA2550066

• Applications: Western Blot
• Synonyms: NF-kappaB p65; NF-kappaB p65 Antibody Sampler Kit; NF-kappaB p65 antibody kit

• Applicable Applications for NF-kappaB p65 antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of MCF7 cells using NFkappaB-p65 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of various cells with Phospho-NFkappaB-p65 (Ser276) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-NFkappaB-p65 (Ser468) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of COS7 cells with Phospho-NFkappaB-p65 (Ser529) Polyclonal Antibody at dilution of 1:500)

Western Blot (WB)

(Western Blot analysis of COLO205 cells with Phospho-NFkappaB-p65 (Ser536) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of Hela cells using NFkappaB-p65 (Acetyl Lys310) Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of Jurkat, K562 cells using NFkappaB-p65 (Acetyl Lys218) Polyclonal Antibody at dilution of 1:1000.)

Related Product Information for NF-kappaB p65 antibody kit

Background: Transcription factors of the nuclear factor kappaB (NF-kappaB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-kappaB1 (p105/p50), and NF- kappaB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kappaB is sequestered in the cytoplasm by IkappaB inhibitory proteins. NF-kappaB-activating agents can induce the phosphorylation of IkappaB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kappaB to enter the nucleus where it regulates gene expression. NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kappaB2 (p100) to produce p52, which translocates to the nucleus. RelA/p65 is a subunit of the NF- kappaB transcription complex, which plays a crucial role in inflammatory and immune responses. The complex is composed of various homodimeric and heterodimeric Rel family member combinations, the activity of which is modulated by post-translational modifications including phosphorylation and acetylation. p65 phosphorylation by PKA and/or MSK1 at Ser276 allows for increased interaction with the transcriptional coactivator p300/CBP to enhance transcriptional activity. NF-kappaB dimer assembly with IkappaB, as well as its DNA binding and transcriptional activities, are regulated by p300/CBP acetyltransferases that principally target Lys218, Lys221 and Lys310. This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-kappaB. T-cell co-stimulation and Calyculin A have both been shown to increase Ser468 phosphorylation. IKKbeta (but not IKKalpha) and GSK-3beta both target this site, which appears to have a negative regulatory role not involving inhibition of nuclear translocation after TNF- alpha or IL-1beta stimulation. p65 phosphorylation at Ser536 regulates activation, nuclear localization, protein-protein interactions, transcriptional activity, and apoptosis.