Akt antibody kit

Phospho-Akt Pathway Antibody Sampler Kit

Cat. Num. AAA2549982

• Applications: Western Blot
• Synonyms: Akt; Phospho-Akt Pathway Antibody Sampler Kit; Akt antibody kit

• Sequence Length: 477

• Applicable Applications for Akt antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of 293T cells using Pan-Akt Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of MCF7 cells with Phospho-Akt (Ser473) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of COLO205 cells with Phospho-Akt (Thr308) Polyclonal Antibody at dilution of 1:500)

Western Blot (WB)

(Western Blot analysis of COS7 cells with Phospho-Raf-1 (Ser259) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of various cells with Phospho-GSK3alpha/beta (Tyr279/216) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-GSK3beta (Ser9) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of Hela cells with Phospho-PTEN (Ser370) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of 1) RAW2647, 2) AD293T cells with Phospho-PTEN (Ser380/T382/T383) Polyclonal Antibody at dilution of 1:1000)

Related Product Information for Akt antibody kit

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin- insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

NCBI and Uniprot Product Information

• NCBI GI #: 1285295799
• NCBI Official Full Name: Akt
• Protein Family: AKT-interacting protein