Western Blot (WB)
(Western blot analysis FoxO3a (Phospho-Ser574) using TNF-a treated 293 whole cell lysates)
Rabbit FoxO3a Polyclonal Antibody | anti-FoxO3a antibody
FoxO3a (Phospho-Ser574) Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
FoxO3a; Polyclonal Antibody; FoxO3a (Phospho-Ser574) Antibody; AF6q21 antibody; AF6q21 protein antibody; DKFZp781A0677 antibody; FKHR2 antibody; FKHRL 1 antibody; FKHRL1 antibody; FKHRL1P2 antibody; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody; Forkhead box O3 antibody; Forkhead box O3A antibody; Forkhead box protein O3 antibody; Forkhead box protein O3A antibody; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody; Forkhead homolog (rhabdomyosarcoma) like 1 antibody; Forkhead in rhabdomyosarcoma like 1 antibody; Forkhead in rhabdomyosarcoma-like 1 antibody; FOX O3A antibody; FOXO2 antibody; foxo3 antibody; FOXO3_HUMAN antibody; FOXO3A antibody; MGC12739 antibody; MGC31925 antibody; anti-FoxO3a antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-FoxO3a (S574) Antibody detects endogenous levels of FoxO3a only when phosphorylated at S574
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (varies by lot)
Sequence Length
673
Applicable Applications for anti-FoxO3a antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
A synthesized peptide derived from human FoxO3a (Phospho-Ser574)
Immunogen Type
Peptide-KLH
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis FoxO3a (Phospho-Ser574) using TNF-a treated 293 whole cell lysates)
Western Blot (WB)
(Western blot analysis FoxO3a (Phospho-Ser574) using TNF-a treated 293 whole cell lysates)
Related Product Information for anti-FoxO3a antibody
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-â-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). p38 phosphorlyates FoxO3a at Ser7 and promotes its nuclear localization (10).
Product Categories/Family for anti-FoxO3a antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97
NCBI Official Full Name
forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
NCBI Protein Information
forkhead box protein O3
UniProt Protein Name
Forkhead box protein O3
UniProt Gene Name
FOXO3
NCBI Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Research Articles on FoxO3a
Similar Products
Product Notes
The FoxO3a foxo3 (Catalog #AAA9429026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FoxO3a (Phospho-Ser574) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FoxO3a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the FoxO3a foxo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FoxO3a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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