Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells transfected with empty vector (D) or different HA-fusion proteins (A, B, C) using HA-Tag Rabbit pAb (1:1000 diluted).)
Rabbit anti-Transfected HA-tag Polyclonal Antibody | anti-HA antibody
Anti-HA-tag Rabbit pAb
Reactivity
Transfected
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
HA-tag; Polyclonal Antibody; Anti-HA-tag Rabbit pAb; HA epitope tag; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain; anti-HA antibody
Host
Rabbit
Reactivity
Transfected
Clonality
Polyclonal
Isotype
Ig
Purity/Purification
Affinity Purified
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Concentration
1.28mg/ml (varies by lot)
Sequence Length
10
Applicable Applications for anti-HA antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
Immunogen
HA synthetic peptide (YPYDVPDYA)conjugated to KLH.
Sensitivity
Transfected Only.
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells transfected with empty vector (D) or different HA-fusion proteins (A, B, C) using HA-Tag Rabbit pAb (1:1000 diluted).)
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells transfected with empty vector (D) or different HA-fusion proteins (A, B, C) using HA-Tag Rabbit pAb (1:1000 diluted).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of CHO K1 cell lysates transfected with HA-tagged fusion protein using HA-Tag Rabbit pAb (301113, 1:100 diluted). HA-Tag mouse mAb (201113) was used for the western blot analysis (1:1000 diluted).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of CHO K1 cell lysates transfected with HA-tagged fusion protein using HA-Tag Rabbit pAb (301113, 1:100 diluted). HA-Tag mouse mAb (201113) was used for the western blot analysis (1:1000 diluted).)
Related Product Information for anti-HA antibody
Entrez Summary: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.Because of their small size, they are unlikely to affect the tagged protein?s biochemical properties.The YPYDVPDYA peptide has been used extensively as a general epitope tag in expression vectors.This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
Product Categories/Family for anti-HA antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HA (Catalog #AAA475562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HA-tag Rabbit pAb reacts with Transfected and may cross-react with other species as described in the data sheet. AAA Biotech's HA-tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000. Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA-tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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