Immunoprecipitation (IP)
(IP antibody use 5ug HA Mouse IgG1 per ml Lysate,WB 1:5000 1. untransfected 293 cell lysate 2. transfected 293 cell lysate with HA-tag fusion protein 3. IP (untransfted 293+anti-HA mAb+Protein G agarose) 4. IP (transfected 293+ normal Mouse IgG+Protein G ag)
Mouse HA-Tag Monoclonal Antibody | anti-HA-Tag antibody
HA-Tag Mouse mAb
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Synonyms
HA-Tag; Monoclonal Antibody; HA-Tag Mouse mAb; anti-HA-Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Form/Format
PBS with 0.02% sodium azide and 50% glycerol pH7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HA-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
WB: 1:5,000
IP: 1:200
IF: 1:1,000
IP: 1:200
IF: 1:1,000
Immunogen
Synthetic Peptide
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(IP antibody use 5ug HA Mouse IgG1 per ml Lysate,WB 1:5000 1. untransfected 293 cell lysate 2. transfected 293 cell lysate with HA-tag fusion protein 3. IP (untransfted 293+anti-HA mAb+Protein G agarose) 4. IP (transfected 293+ normal Mouse IgG+Protein G ag)
Immunoprecipitation (IP)
(IP antibody use 5ug HA Mouse IgG1 per ml Lysate,WB 1:5000 1. untransfected 293 cell lysate 2. transfected 293 cell lysate with HA-tag fusion protein 3. IP (untransfted 293+anti-HA mAb+Protein G agarose) 4. IP (transfected 293+ normal Mouse IgG+Protein G ag)
Immunofluorescence (IF)
(IF analysis of 293 cells transfected with a HA-tag protein, using anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA))
Immunofluorescence (IF)
(IF analysis of 293 cells transfected with a HA-tag protein, using anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA))
Testing Data
(0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000.)
Testing Data
(0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000.)
Related Product Information for anti-HA-Tag antibody
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.
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Product Notes
The HA-Tag (Catalog #AAA8808473) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HA-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:5,000 IP: 1:200 IF: 1:1,000. Researchers should empirically determine the suitability of the HA-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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