Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acid Phosphatase (ACP) Standard Curve.)
Mouse Acid Phosphatase (ACP) ELISA Kit | ACP elisa kit
Mouse Acid Phosphatase (ACP) ELISA Kit
Gene Names
Acp2; LAP; Acp-2
Reactivity
Mouse
Synonyms
Acid Phosphatase (ACP); Mouse Acid Phosphatase (ACP) ELISA Kit; ACP2; ACP elisa kit
Reactivity
Mouse
Specificity
Mouse Acid Phosphatase (ACP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACP2. No significant cross-reactivity or interference between Mouse ACP2 and analogues was observed.
Sequence Length
423
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acid Phosphatase (ACP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acid Phosphatase (ACP) Standard Curve.)
Related Product Information for ACP elisa kit
Description: This Mouse Acid Phosphatase (ACP) ELISA Kit employs a two-site sandwich ELISA to quantitate ACP2 in samples. An antibody specific for ACP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The acyl carrier protein (ACP) is an important component in both fatty acid and polyketide biosynthesis with the growing chain bound during synthesis as a thiol ester at the distal thiol of a 4'-phosphopantethiene moiety. The protein is expressed in the inactive apo form and the 4'-phosphopantetheine moiety must be post-translationally attached to a conserved serine residue on the ACP by the action of holo-acyl carrier protein synthase (ACPS) a phosphopantetheinyl transferase.The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria.Runswick et al. (1991) reported that the bovine SDAP subunit of complex I is an acyl carrier protein. Loeffen et al. (1998) stated that GenBank represents the cDNA encoding the human homolog of SDAP.
Background: The acyl carrier protein (ACP) is an important component in both fatty acid and polyketide biosynthesis with the growing chain bound during synthesis as a thiol ester at the distal thiol of a 4'-phosphopantethiene moiety. The protein is expressed in the inactive apo form and the 4'-phosphopantetheine moiety must be post-translationally attached to a conserved serine residue on the ACP by the action of holo-acyl carrier protein synthase (ACPS) a phosphopantetheinyl transferase.The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria.Runswick et al. (1991) reported that the bovine SDAP subunit of complex I is an acyl carrier protein. Loeffen et al. (1998) stated that GenBank represents the cDNA encoding the human homolog of SDAP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lysosomal acid phosphatase isoform 1
NCBI Official Synonym Full Names
acid phosphatase 2, lysosomal
NCBI Official Symbol
Acp2
NCBI Official Synonym Symbols
LAP; Acp-2
NCBI Protein Information
lysosomal acid phosphatase
UniProt Protein Name
Lysosomal acid phosphatase
Protein Family
UniProt Gene Name
Acp2
UniProt Synonym Gene Names
LAP
NCBI Description
The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017]
Research Articles on ACP
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Product Notes
The Mouse ACP acp2 (Catalog #AAA9718168) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718168 ELISA Kit recognizes Mouse ACP. It is sometimes possible for the material contained within the vial of "Acid Phosphatase (ACP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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