Human Angiopoietin-2 ELISA Kit | ANGPT2 elisa kit

ANGII Human ELISA Kit

Cat. Num. AAA355302

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human
• Applications: ELISA
• Synonyms: Angiopoietin-2; ANGII Human ELISA Kit; Human Angiopoietin-2; ANGPT2 elisa kit

• Reactivity: Human

• Applicable Applications for ANGPT2 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of ANGII in Human serum, plasma, urine, cell culture supernatant or tissue samples.
• Assay Type: Sandwich
• Detection Range: 8 pg/ml-150 pg/ml
• Preparation and Storage: Store at 2-8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPT2 elisa kit

Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-ANG II antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-ANG II antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG II amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG II can be calculated.

Background: Angiopoietins are protein growth factors that promote angiogenesis, the formation of blood vessels from pre-existing blood vessels. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. ANG II is a naturally occurring antagonist of angiopoietin-1 (ANGPT1) that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. ANG II is a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema. It plays a critical role in inducing tumor cell infiltration, and that this invasive phenotype is caused by activation of MMP2.

NCBI and Uniprot Product Information

• NCBI GI #: 50082538
• NCBI GeneID: 285
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 56,919 Da
• NCBI Official Full Name: angiopoietin-2
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Ref.1 Ref.9 Ref.10 Ref.11

Subunit structure: Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. Ref.1 Ref.8 Ref.9 Ref.11

Subcellular location: Secreted.

Domain: The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

Sequence similarities: Contains 1 fibrinogen C-terminal domain.