Flow Cytometry (FC/FACS)
Rabbit Angiopoietin-2/ANGPT2 Polyclonal Antibody | anti-ANGPT2 antibody
Anti-Angiopoietin-2/ANGPT2 Antibody
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunofluorescence (IF)
Immunofluorescence (IF)
Western Blot (WB)
(Figure 1. Western blot analysis of ANGPT2 using anti-ANGPT2 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of ANGPT2 using anti-ANGPT2 antibody.)
Background: ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
2. Grosios, K., Leek, J. P., Markham, A. F., Yancopoulos, G. D., Jones, P. F. Assignment of ANGPT4, ANGPT1, and ANGPT2 encoding Angiopoietins 4, 1 and 2 to human chromosome bands 20p13, 8q22.3-q23 and 8p23.1, respectively, by in situ hybridization and radiation hybrid mapping. Cytogenet. Cell Genet. 84: 118-120, 1999.
3. Holash, J., Maisonpierre, P. C., Compton, D., Boland, P., Alexander, C. R., Zagzag, D., Yancopoulos, G. D., Wiegand, S. J. Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. Science 284: 1994-1998, 1999.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an antagonist of angiopoietin 1 (Ang-1), and both (Ang-1 and Ang-2) are ligands for the endothelial tyrosine kinase receptor (Tie2). These two ligands affect angiogenesis during embryogenesis and tumorigenesis. The encoded protein disrupts the vascular remodeling ability of Ang-1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Nov 2019]
Uniprot Description
ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus
Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding
Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; negative regulation of blood vessel endothelial cell migration; signal transduction; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; response to radiation; negative regulation of angiogenesis; positive regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; response to activity; angiogenesis; blood coagulation; leukocyte migration
Research Articles on ANGPT2
Similar Products
Product Notes
The ANGPT2 angpt2 (Catalog #AAA1752927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Angiopoietin-2/ANGPT2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Angiopoietin-2/ANGPT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Human, Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human) ICC: 2ug/ml (Human) IF: 2ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the ANGPT2 angpt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin-2/ANGPT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
