Mouse AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit | AGER clia kit

Mouse AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit

Cat. Num. AAA2533008

• Gene Names: Ager; RAGE
• Reactivity: Mouse
• Synonyms: AGER (Advanced Glycosylation End Product Specific Receptor); Mouse AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit; RAGE; AGER clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant AGER. No significant cross-reactivity or interference between AGER and analogues was observed.
• Sequence Length: 402

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGER clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of AGER concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to AGER. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AGER and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of AGER. You can calculate the concentration of AGER in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 259089426
• NCBI GeneID: 81722
• NCBI Accession #: NP_445788.2
• NCBI GenBank Nucleotide #: NM_053336.2
• Molecular Weight: 42,664 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage
• UniProt Entry Name: RAGE_RAT

NCBI Description

cell surface receptor for various molecules, including the amyloidogenic form of serum amyloid A [RGD, Feb 2006]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell surface; cell soma; axon; cytoplasm; extracellular region; integral to membrane; basal plasma membrane; plasma membrane; cytosol

Molecular Function: heparin binding; identical protein binding; protein binding; advanced glycation end-product receptor activity

Biological Process: positive regulation of JNK activity; glycoprotein metabolic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of smooth muscle cell proliferation; negative regulation of cell adhesion; positive regulation of smooth muscle cell migration; response to vitamin A; induction of positive chemotaxis; activation of NF-kappaB transcription factor; calcium ion homeostasis; connective tissue replacement during inflammatory response; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; regulation of DNA binding; positive regulation of neuron apoptosis; response to methylglyoxal; inflammatory response; neurite development; positive regulation of autophagy; response to fructose stimulus; JAK-STAT cascade; astrocyte development; response to genistein; response to hyperoxia; regulation of inflammatory response; negative regulation of endothelial cell proliferation; response to hypoxia; negative regulation of osteoblast proliferation; response to activity; positive regulation of protein amino acid phosphorylation; brain development; positive regulation of phagocytosis, engulfment; lung development; positive regulation of inflammatory response; positive regulation of cell migration