Typical Testing Data/Standard Curve (for reference only)
(Human Rage PicoKine ELISA Kit standard curve )
Human Rage ELISA Kit | AGER elisa kit
Human Rage PicoKine ELISA Kit
Gene Names
AGER; RAGE
Reactivity
Human
Synonyms
Rage; Human Rage PicoKine ELISA Kit; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor; Advanced glycosylation end product specific receptor; Ager; DAMA 358M23.4; LE 9211 A antigen ; MAPK MAK MRK overlapping kinase ; MGC22357; MOK; OTTHUMP00000174885; RAGE 1; RAGE_HUMAN; RAGE1; Receptor for advanced glycation end products; Receptor for advanced glycosylation end products; Renal tumor antigen ; Renal tumor antigen 1; AGER elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Rage
Sequence Length
435
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
78pg/ml-5,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human Rage PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Rage PicoKine ELISA Kit standard curve )
Related Product Information for AGER elisa kit
Principle of the assay: human Rage ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rage has been precoated onto 96-well plates. Standards(NSO, Q24-A344) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rage is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Rage amount of sample captured in plate.
Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as "AGER". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as "AGER". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
45,980 Da
NCBI Official Synonym Full Names
advanced glycosylation end product-specific receptor
NCBI Official Symbol
AGER
NCBI Official Synonym Symbols
RAGE
NCBI Protein Information
advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta; receptor for advanced glycation end-products variant 20
UniProt Protein Name
cDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor
Protein Family
UniProt Entry Name
B4DNX3_HUMAN
NCBI Description
The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]
Research Articles on AGER
Similar Products
Product Notes
The Human AGER (Catalog #AAA175971) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175971 ELISA Kit recognizes Human AGER. It is sometimes possible for the material contained within the vial of "Rage, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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