Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit | AGER elisa kit

Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit

Cat. Num. AAA2023573

• Gene Names: Ager; RAGE
• Reactivity: Human
• Synonyms: Advanced Glycosylation End Product Specific Receptor (AGER); Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit; RAGE; Receptor For Advanced Glycation Endproducts; AGER elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Advanced Glycosylation End Product Specific Receptor (AGER).; No significant cross-reactivity or interference between Advanced Glycosylation End Product Specific Receptor (AGER) and analogues was observed.
• Sequence Length: 402

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Advanced Glycosylation End Product Specific Receptor (AGER) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Advanced Glycosylation End Product Specific Receptor (AGER) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGER elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Advanced Glycosylation End Product Specific Receptor (AGER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Advanced Glycosylation End Product Specific Receptor (AGER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Advanced Glycosylation End Product Specific Receptor (AGER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Advanced Glycosylation End Product Specific Receptor (AGER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AGER elisa kit

Metabolic pathway; Tumor immunity; Endocrinology; Cardiovascular biology; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 259089426
• NCBI GeneID: 81722
• NCBI Accession #: NP_445788.2
• NCBI GenBank Nucleotide #: NM_053336.2
• UniProt Accession #: Q63495
• Molecular Weight: 42,664 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage
• UniProt Entry Name: RAGE_RAT

NCBI Description

cell surface receptor for various molecules, including the amyloidogenic form of serum amyloid A [RGD, Feb 2006]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis; Cell cycle regulation

Cellular Component: extracellular space; cell surface; cell soma; axon; cytoplasm; integral to membrane; plasma membrane; basal plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; advanced glycation end-product receptor activity

Biological Process: positive regulation of JNK activity; glycoprotein metabolic process; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; negative regulation of collagen biosynthetic process; negative regulation of cell adhesion; positive regulation of smooth muscle cell migration; induction of positive chemotaxis; activation of NF-kappaB transcription factor; response to vitamin A; calcium ion homeostasis; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; regulation of DNA binding; positive regulation of neuron apoptosis; response to methylglyoxal; inflammatory response; positive regulation of autophagy; neurite development; response to fructose stimulus; JAK-STAT cascade; response to genistein; response to hyperoxia; regulation of inflammatory response; negative regulation of endothelial cell proliferation; response to hypoxia; negative regulation of osteoblast proliferation; response to activity; positive regulation of protein amino acid phosphorylation; brain development; positive regulation of phagocytosis, engulfment; lung development; positive regulation of cell migration; positive regulation of inflammatory response