Rabbit anti-Human PKC epsilon Polyclonal Antibody | anti-PKCepsilon antibody

PKC epsilon (Phospho-S729) Polyclonal Antibody

Cat. Num. AAA3005074

• Gene Names: PRKCE; PKCE; nPKC-epsilon
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PKC epsilon; Polyclonal Antibody; PKC epsilon (Phospho-S729) Polyclonal Antibody; Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE; anti-PKCepsilon antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKC epsilon (phospho-S729) polyclonal antibody detects endogenous levels of PKC epsilon protein only when phosphorylated at Ser729.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 737

• Applicable Applications for anti-PKCepsilon antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human PKC epsilon around the phosphorylation site of Serine 729
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -22 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PKC epsilon (phospho-S729) polyclonal antibody at 1:500 dilutionLane1:HEK293 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:SGC7901 treated with PMA (50ng/ml) for 5 minutes then repair for 10 minutes whole cell lysate (40ug) Lane5:SGC7901 treated with PMA (50ng/ml) for 5 minutes then repair for 30 minutes whole cell lysate (40ug))

Related Product Information for anti-PKCepsilon antibody

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (alpha, betaI, betaII, gamma, delta, epsilon, omega, eta, tau, lambda, upsilon and nu). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC delta and epsilon are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

NCBI and Uniprot Product Information

• NCBI GI #: 4885563
• NCBI GeneID: 5581
• NCBI Accession #: NP_005391.1
• NCBI GenBank Nucleotide #: NP_005391.1
• UniProt Accession #: Q02156
• Molecular Weight: ~ 76~85kDa
• NCBI Official Full Name: protein kinase C epsilon type
• NCBI Official Synonym Full Names: protein kinase C epsilon
• NCBI Official Symbol: PRKCE
• NCBI Official Synonym Symbols: PKCE; nPKC-epsilon
• NCBI Protein Information: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• UniProt Gene Name: PRKCE
• UniProt Synonym Gene Names: PKCE
• UniProt Entry Name: KPCE_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: actin monomer binding; calcium-independent protein kinase C activity; protein serine/threonine kinase activity; signal transducer activity; protein binding; protein kinase C activity; enzyme binding; metal ion binding; enzyme activator activity; ethanol binding; receptor activator activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; apoptosis; response to morphine; positive regulation of lipid catabolic process; signal transduction; protein amino acid phosphorylation; positive regulation of MAPKKK cascade; lipopolysaccharide-mediated signaling pathway; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; positive regulation of cytokinesis; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of synaptic transmission, GABAergic; positive regulation of insulin secretion; cell cycle; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; cell division; phospholipase C activation; release of sequestered calcium ion into cytosol; macrophage activation during immune response; innate immune response; blood coagulation