Blk, active Active Protein | Bik active protein

Blk, active, Recombinant, Human (B lymphoid kinase, B lymphoid tyrosine kinase)

Cat. Num. AAA636231

• Gene Names: Bik; Blk; Nbk; Biklk
• Purity: Purified; 90% (SDS-PAGE and Coomassie blue staining)
• Synonyms: Blk; active; Recombinant; Human (B lymphoid kinase; B lymphoid tyrosine kinase); Bik active protein

• Purity/Purification: Purified; 90% (SDS-PAGE and Coomassie blue staining)
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

• Specific Activity: 579nmol phosphate incorporated into Poly(Glu-Tyr) per minute/mg protein at 30ºC using a final concentration of 50uM ATP (0.83uCi/assay).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for Bik active protein

Blk, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells (1). Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk (2). Upon activation, the SH2 domain of Blk binds more than 10 distinct phosphoprotein including those of 72, 76, 115, and 130 kd (3). Blk prefers peptide substrates of the structure I/L-Y-D/E-X-L which resemble critical features of the ITAM motifs found in, e.g. the intracellular components Ig-alpha and Ig-beta of the beta cell receptor. Blk has a strong preference for a negatively charged amino acid in position +1 (4).

Product Categories/Family for Bik active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6671638
• NCBI GeneID: 12124
• NCBI Accession #: NP_031572.1
• NCBI GenBank Nucleotide #: NM_007546.2
• UniProt Accession #: O70337
• Molecular Weight: ~84kD
• NCBI Official Full Name: bcl-2-interacting killer
• NCBI Official Synonym Full Names: BCL2-interacting killer
• NCBI Official Symbol: Bik
• NCBI Official Synonym Symbols: Blk; Nbk; Biklk
• NCBI Protein Information: bcl-2-interacting killer; apoptosis inducer NBK; bik-like killer protein
• UniProt Protein Name: Bcl-2-interacting killer
• Protein Family: Bcl-2-interacting killer
• UniProt Gene Name: Bik
• UniProt Synonym Gene Names: Biklk; Blk; Nbk
• UniProt Entry Name: BIK_MOUSE

Uniprot Description

BIK: accelerates programmed cell death. Binding to the apoptosis repressors Bcl-xL, Bcl-2, or BHRF1 (the Epstein-Barr virus homolog of Bcl-2) suppresses its death-promoting activity. Contains 1 Bcl-2 homology 3 (BH3) domain. Does not interact with Bax.

Protein type: Mitochondrial; Apoptosis; Membrane protein, integral

Cellular Component: mitochondrial envelope; mitochondrion; membrane; integral to membrane

Molecular Function: BH domain binding; protein binding; protein heterodimerization activity

Biological Process: apoptotic mitochondrial changes; positive regulation of protein homooligomerization; apoptosis; male gonad development; spermatogenesis