Testing Data
(The approximate molecular weight is 44kD and the average purity is 90%.)
ERK1, active Active Protein
ERK1, active, Recombinant, Human (Extracellular Signal-regulated Kinase 1, ERK-1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, MAPK3, Mitog
Purity
Highly Purified90% by densitometry
Synonyms
ERK1; active; Recombinant; Human (Extracellular Signal-regulated Kinase 1; ERK-1; ERT2; Insulin-stimulated MAP2 Kinase; MAP Kinase Isoform p44; Microtubule-associated Protein 2 Kinase; Mitogen-activated Protein Kinase 3; MAP Kinase 3; MAPK 3; MAPK3; Mitog; active active protein
Host
Baculovirus in Sf9 insect cells
Purity/Purification
Highly Purified
90% by densitometry
90% by densitometry
Form/Format
Supplied as a liquid in 25mM MOPS, pH 7.5, 300mM sodium chloride, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Activity
The specific activity of ERK1 was determined to be 330nmol/min/mg using a myelin basic protein (MBP) substrate (see activity assay protocol).
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(The approximate molecular weight is 44kD and the average purity is 90%.)
Testing Data
(The approximate molecular weight is 44kD and the average purity is 90%.)
Related Product Information for ERK1, active active protein
ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.
Recombinant protein corresponding to full-length human ERK1expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro (NM_002746).
Recombinant protein corresponding to full-length human ERK1expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro (NM_002746).
Product Categories/Family for ERK1, active active protein
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Product Notes
The ERK1, active (Catalog #AAA636111) is an Active Protein produced from Baculovirus in Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ERK1, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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