Testing Data
(Akt3 activity assay)
AKT3, active Active Protein
AKT3, active, Recombinant, Human (RAC-gamma Serine/Threonine-protein Kinase, RAC-PK-gamma, Protein Kinase Akt-3, Protein Kinase B gamma, PKB gamma, PKBG, STK-2)
Purity
Highly Purified~92% (SDS-PAGE)
Synonyms
AKT3; active; Recombinant; Human (RAC-gamma Serine/Threonine-protein Kinase; RAC-PK-gamma; Protein Kinase Akt-3; Protein Kinase B gamma; PKB gamma; PKBG; STK-2); active active protein
Purity/Purification
Highly Purified
~92% (SDS-PAGE)
~92% (SDS-PAGE)
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Specific Activity
206nmol/min/mg
Biological Activity
206nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30 degree C for 15 minutes using a final concentration of 50uM ATP and total of 0.83uCi/ul P-32.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Akt3 activity assay)
Testing Data
(Akt3 activity assay)
Testing Data
(Akt3 protein gel)
Testing Data
(Akt3 protein gel)
Related Product Information for AKT3, active active protein
Akt3 or Protein Kinase B g (PKB) is a serine/threonine kinase that is a member of the Akt family. In mammals, the Akt family comprises of three highly homologous members known as Akt1 (PKB), Akt2 (PKB), and Akt 3. Akt 3 like the other family members is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The activation mechanism remains to be fully characterized but occurs downstream of phosphoinositide 3-kinase (PI3K). PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt family members to the plasma membrane where they are phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) and phosphoinositide-dependent kinase-2 (PDK-2 possibly ILK). Akt 3 like the other family members phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that Akt 3 is frequently overexpressed in many types of human cancers including breast and prostate. Although the mechanisms have not yet been fully characterized, increased expression and activation of Akt is believed to promote cell proliferation and survival thereby contributing to cancer progression.
Product Categories/Family for AKT3, active active protein
Similar Products
Product Notes
The AKT3, active (Catalog #AAA637364) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AKT3, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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