Testing Data
(Protein Concentration (ng/assay) of S1010-81E.)
SGK, active, truncated NT 60aa Active Protein
SGK, active, truncated NT 60aa, Recombinant, Human (SGK1, Serum and Glucocorticoid-regulated Protein Kinase)
Purity
Highly Purified90% as determined by SDS-PAGE analysis.
Synonyms
SGK; active; truncated NT 60aa; Recombinant; Human (SGK1; Serum and Glucocorticoid-regulated Protein Kinase); truncated NT 60aa active protein
Purity/Purification
Highly Purified
90% as determined by SDS-PAGE analysis.
90% as determined by SDS-PAGE analysis.
Form/Format
Supplied in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Specific Activity
~60nmol/min/mg
Activity Assay:
Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 degree C using a final concentration of 50uM [32P] ATP.
Activity Assay:
Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 degree C using a final concentration of 50uM [32P] ATP.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Protein Concentration (ng/assay) of S1010-81E.)
Testing Data
(Protein Concentration (ng/assay) of S1010-81E.)
SDS-Page
(SDS-PAGE of S1010-81E.)
SDS-Page
(SDS-PAGE of S1010-81E.)
Related Product Information for SGK, active, truncated NT 60aa active protein
SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family. SGK1 is activated in vitro by 3-phosphoinositide- dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1, mRNA is
Product Categories/Family for SGK, active, truncated NT 60aa active protein
Similar Products
Product Notes
The SGK, active, truncated NT 60aa (Catalog #AAA636873) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SGK, active, truncated NT 60aa, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
