Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit | ABCC4 elisa kit

Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit

Cat. Num. AAA456184

• Gene Names: ABCC4; MRP4; MOATB; MOAT-B
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter C4 (ABCC4); Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit; ABCC4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ABCC4. No significant cross-reactivity or interference between ABCC4 and analogues was observed.
• Sequence Length: 784

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.118ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ABCC4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ABCC4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCC4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ABCC4 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABCC4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ABCC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ABCC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ABCC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157502203
• NCBI GeneID: 10257
• NCBI Accession #: NP_001098985.1
• NCBI GenBank Nucleotide #: NM_001105515.2
• UniProt Accession #: O15439; Q8IVZ4; Q8IZN6; Q8NEW8; Q9Y6J2; A9Z1Z7; B7Z3Q7
• Molecular Weight: 88,138 Da
• NCBI Official Full Name: multidrug resistance-associated protein 4 isoform 2
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 4
• NCBI Official Symbol: ABCC4
• NCBI Official Synonym Symbols: MRP4; MOATB; MOAT-B
• NCBI Protein Information: multidrug resistance-associated protein 4
• UniProt Protein Name: Multidrug resistance-associated protein 4
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC4
• UniProt Synonym Gene Names: MRP4; MOAT-B

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

May be an organic anion pump relevant to cellular detoxification.