Rabbit ABCC4 Polyclonal Antibody | anti-ABCC4 antibody

ABCC4 Antibody

Cat. Num. AAA2518467

• Gene Names: ABCC4; MRP4; MOATB; MOAT-B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: ABCC4; Polyclonal Antibody; ABCC4 Antibody; EST170205; MOAT-B; MOATB; MRP4; anti-ABCC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 859

• Applicable Applications for anti-ABCC4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant protein of human ABCC4
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5767 / sc-5765 / sc-20768
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ABCC4 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human esophageal cancer using ABCC4 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using ABCC4 antibody at dilution of 1:200 (200x lens).)

Related Product Information for anti-ABCC4 antibody

ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family. ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis. There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White (1). ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4. ABCC4 is widely expressed in cells and tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines (2-4). ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds (5). The diversity of substrates determines the biological functions of ABCC4. It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells (6). ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostagladins (7). Researchers have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy (8, 9). ABCC4 localizes to both plasma membrane and intracellular membranous structures (10). Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki desease, a genetic childhood disease characterized by vasculitis (11).

NCBI and Uniprot Product Information

• NCBI GI #: 27371328
• NCBI GeneID: 10257
• Molecular Weight: 88,138 Da
• NCBI Official Full Name: ABCC4 protein
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 4
• NCBI Official Symbol: ABCC4
• NCBI Official Synonym Symbols: MRP4; MOATB; MOAT-B
• NCBI Protein Information: multidrug resistance-associated protein 4; MRP/cMOAT-related ABC transporter; bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4); canalicular multispecific organic anion transporter (ABC superfamily); multi-specific organic anion transporter B
• UniProt Protein Name: Multidrug resistance-associated protein 4
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC4
• UniProt Synonym Gene Names: MRP4; MOAT-B
• UniProt Entry Name: MRP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Protein type: Membrane protein, integral; Transporter; Transporter, ABC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: membrane; basolateral plasma membrane; plasma membrane; integral to membrane; platelet dense granule membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; ATP binding

Biological Process: response to drug; platelet activation; platelet degranulation; positive regulation of smooth muscle cell proliferation; blood coagulation; response to organic nitrogen; transmembrane transport; response to organic cyclic substance