Western Blot (WB)
(CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).)
Rabbit anti-Human CPSF3L Polyclonal Antibody | anti-CPSF3L antibody
CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD) (Biotin)
Gene Names
CPSF3L; RC68; INT11; RC-68; INTS11; CPSF73L; FLJ13294; FLJ20542
Reactivity
Human
Applications
Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CPSF3L; Polyclonal Antibody; NT (CPSF3L; INTS11; RC68; Integrator complex subunit 11; Cleavage and polyadenylation-specific factor 3-like protein; Protein related to CPSF subunits of 68kD) (Biotin); Protein related to CPSF subunits of 68kD; anti-CPSF3L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-CPSF3L antibody
Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CPSF3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the N-terminal region of human CPSF3L.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).)
Western Blot (WB)
(CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).)
Immunohistochemistry (IHC)
(CPSF3L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPSF3L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CPSF3L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPSF3L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CPSF3L Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CPSF3L Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-CPSF3L antibody
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).
Product Categories/Family for anti-CPSF3L antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Baillat, D., et al. Cell 123(2):265-276(2005). Dominski, Z., et al. Mol. Cell. Biol. 25(4):1489-1500(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,663 Da
NCBI Official Full Name
integrator complex subunit 11
NCBI Official Synonym Full Names
cleavage and polyadenylation specific factor 3-like
NCBI Official Symbol
CPSF3L
NCBI Official Synonym Symbols
RC68; INT11; RC-68; INTS11; CPSF73L; FLJ13294; FLJ20542
NCBI Protein Information
integrator complex subunit 11; CPSF3-like protein; OTTHUMP00000003390; OTTHUMP00000003391; OTTHUMP00000003393; OTTHUMP00000003397; related to CPSF subunits 68 kDa; protein related to CPSF subunits of 68 kDa; cleavage and polyadenylation-specific factor 3-
UniProt Protein Name
Integrator complex subunit 11
UniProt Gene Name
CPSF3L
UniProt Synonym Gene Names
INTS11; RC68; Int11; CPSF3-like protein; RC-68
UniProt Entry Name
INT11_HUMAN
Uniprot Description
INTS11: Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA processing; Ribonuclease; EC 3.1.27.-
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: cytoplasm; integrator complex
Molecular Function: protein binding; hydrolase activity
Biological Process: snRNA processing
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Product Notes
The CPSF3L cpsf3l (Catalog #AAA6288419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPSF3L can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CPSF3L cpsf3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPSF3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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