Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Meprin A subunit beta (MEP1B) Standard Curve.)
Mouse Meprin A Subunit beta (MEP1B) ELISA Kit | MEP1B elisa kit
Mouse Meprin A Subunit beta (MEP1B) ELISA Kit
Gene Names
Mep1b; Mep-1b
Reactivity
Mouse
Synonyms
Meprin A Subunit beta (MEP1B); Mouse Meprin A Subunit beta (MEP1B) ELISA Kit; MEP1B; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; PABA peptide hydrolase; PPH beta; endopeptidase-2; meprin A beta; MEP1B elisa kit
Reactivity
Mouse
Specificity
Mouse Meprin A subunit beta (MEP1B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MEP1B. No significant cross-reactivity or interference between Mouse MEP1B and analogues was observed.
Sequence Length
704
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Meprin A subunit beta (MEP1B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Meprin A subunit beta (MEP1B) Standard Curve.)
Related Product Information for MEP1B elisa kit
Description: This Mouse Meprin A subunit beta (MEP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MEP1B in samples. An antibody specific for MEP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. MEP1B encodes the beta subunit. Targeted disruption of MEP1B in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
Background: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. MEP1B encodes the beta subunit. Targeted disruption of MEP1B in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
meprin A subunit beta
NCBI Official Synonym Full Names
meprin 1 beta
NCBI Official Symbol
Mep1b
NCBI Official Synonym Symbols
Mep-1b
NCBI Protein Information
meprin A subunit beta
UniProt Protein Name
Meprin A subunit beta
Protein Family
UniProt Gene Name
Mep1b
UniProt Synonym Gene Names
Mep-1b
Uniprot Description
MEP1B: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]
Protein type: EC 3.4.24.63; Membrane protein, integral; Protease
Chromosomal Location of Human Ortholog: 18 A2|18 11.81 cM
Cellular Component: membrane
Molecular Function: metalloendopeptidase activity
Research Articles on MEP1B
Similar Products
Product Notes
The Mouse MEP1B mep1b (Catalog #AAA9717666) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717666 ELISA Kit recognizes Mouse MEP1B. It is sometimes possible for the material contained within the vial of "Meprin A Subunit beta (MEP1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
