Meprin beta Recombinant Protein | MEP1B recombinant protein

Meprin beta Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8120448

• Gene Names: MEP1B; MEP1B
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Meprin beta; Meprin beta Protein; Human; Recombinant (ECD; His Tag); Human MEP1B/Meprin beta Protein (ECD; meprin A; beta; MEP1B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr652

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human MEP1B (NP_005916.2)(Met1-Thr652) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MEP1B recombinant protein

Background: MEP1B (Meprin A Subunit Beta) is a Protein Coding gene. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in the kidney and intestine. Meprins are cell membrane, oligomeric metalloendopeptidases composed of two distinct but evolutionarily related subunits, alpha, and beta. MEP1A is mapped to the short arm of chromosome 6 by the use of radiation and somatic cell hybrids. More specifically, it is localized between the centromere and GSTA2 in 6p11-p12. MEP1B mapped to chromosome 18, by the use of somatic cell hybrids, in 18q12.2-q12.3, proximal to the TTR/PALB gene. Diseases associated with MEP1B include Powassan Encephalitis and Deafness, Autosomal Dominant 31.

NCBI and Uniprot Product Information

• NCBI GI #: 153070264
• NCBI GeneID: 4225
• NCBI Accession #: NP_005916.2
• NCBI GenBank Nucleotide #: NM_005925.2
• UniProt Accession #: Q16820; Q670J1; B7ZM35; B9EGL6
• Molecular Weight: 79,571 Da
• NCBI Official Full Name: meprin A subunit beta
• NCBI Official Synonym Full Names: meprin A, beta
• NCBI Official Symbol: MEP1B
• NCBI Official Synonym Symbols: MEP1B
• NCBI Protein Information: meprin A subunit beta; PPH beta; meprin B; endopeptidase-2; PABA peptide hydrolase; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• UniProt Protein Name: Meprin A subunit beta
• Protein Family: Meprin
• UniProt Gene Name: MEP1B

NCBI Description

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes and in leukocytes and certain cancer cells. Mature meprins are oligomers of evolutionarily related, separately encoded alpha and/or beta subunits. Homooligomers of meprin-alpha (MEP1A; MIM 600388) are secreted; oligomers containing meprin-beta are associated with the plasma membrane. Substrates include bioactive peptides and extracellular matrix proteins. See MIM 600388 for further information on meprins.[supplied by OMIM]

Uniprot Description

Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.