Rabbit anti-Guinea pig Transforming Growth Factor Beta 1 (TGFb1) Polyclonal Antibody | anti-TGFb1 antibody

Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

Cat. Num. AAA2004135

• Gene Names: Tgfb1; TGF-beta
• Reactivity: Guinea pig
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Transforming Growth Factor Beta 1 (TGFb1); Polyclonal Antibody; Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1); anti-TGFb1 antibody

• Host: Rabbit
• Reactivity: Guinea pig
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TGFb1. It has been selected for its ability to recognize TGFb1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 0.82mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- GL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKAKVEQLS NMIVRSCKCS
• Sequence Length: 51

• Applicable Applications for anti-TGFb1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant TGFb1 (Gly279~Ser390) expressed in E.coli.
• Cross Reactivity: Guinea pig
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2038235
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

• NCBI GI #: 290491244
• NCBI GeneID: 100135628
• NCBI Accession #: NP_001166494.1
• NCBI GenBank Nucleotide #: NM_001173023.1
• UniProt Accession #: Q9Z1Y6; Q9QZB3; Q9R148
• Molecular Weight: 44,328 Da
• NCBI Official Full Name: transforming growth factor beta-1
• NCBI Official Symbol: Tgfb1
• NCBI Official Synonym Symbols: TGF-beta
• NCBI Protein Information: transforming growth factor beta-1
• UniProt Protein Name: Transforming growth factor beta-1
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFB1
• UniProt Synonym Gene Names: TGF-beta-1; LAP

Uniprot Description

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types ().