Human Meprin A Beta (MEP1b) RTU ELISA Kit | MEP1b rtu elisa kit

Human Meprin A Beta (MEP1b) ELISA Kit

Cat. Num. AAA4500980

• Reactivity: Human
• Synonyms: Meprin A Beta (MEP1b); Human Meprin A Beta (MEP1b) ELISA Kit; PPH Beta; Endopeptidase-2; Meprin B; PABA Peptide Hydrolase; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Beta; MEP1b rtu elisa kit

• Reactivity: Human
• Sequence Length: 700

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MEP1b rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MEP1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MEP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MEP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MEP1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 815890920
• NCBI GeneID: 4225
• NCBI Accession #: NP_001295100.1
• NCBI GenBank Nucleotide #: NM_001308171.1
• UniProt Accession #: Q16820; Q670J1; B7ZM35; B9EGL6
• Molecular Weight: 79,571 Da
• NCBI Official Full Name: meprin A subunit beta isoform 2
• NCBI Official Synonym Full Names: meprin A subunit beta
• NCBI Official Symbol: MEP1B
• NCBI Protein Information: meprin A subunit beta
• UniProt Protein Name: Meprin A subunit beta
• Protein Family: Meprin
• UniProt Gene Name: MEP1B

NCBI Description

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]

Uniprot Description

Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.