Mouse MEP1B ELISA Kit | MEP1B elisa kit

Mouse MEP1B ELISA Kit

Cat. Num. AAA8248448

• Gene Names: Mep1b; Mep-1b
• Reactivity: Mouse
• Synonyms: MEP1B; Mouse MEP1B ELISA Kit; Meprin A subunit beta; Endopeptidase-2; Meprin B; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta; MEP1B elisa kit

• Reactivity: Mouse
• Specificity: The Mouse MEP1B ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse MEP1B proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 704

• Samples: Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MEP1B elisa kit

Principle of the Assay: The Mouse MEP1B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MEP1B in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Mouse MEP1B coated on a 96-well plate. Standards and samples are pipetted into the wells and MEP1B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse MEP1B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MEP1B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases. Both meprin subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Merpin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only). Although the two meprin subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity.

NCBI and Uniprot Product Information

• NCBI GI #: 147901863
• NCBI GeneID: 17288
• NCBI Accession #: NP_032612.2
• NCBI GenBank Nucleotide #: NM_008586.2
• UniProt Accession #: Q61847; Q059K0
• Molecular Weight: 80,348 Da
• NCBI Official Full Name: meprin A subunit beta
• NCBI Official Synonym Full Names: meprin 1 beta
• NCBI Official Symbol: Mep1b
• NCBI Official Synonym Symbols: Mep-1b
• NCBI Protein Information: meprin A subunit beta
• UniProt Protein Name: Meprin A subunit beta
• Protein Family: Meprin
• UniProt Gene Name: Mep1b
• UniProt Synonym Gene Names: Mep-1b
• UniProt Entry Name: MEP1B_MOUSE

Uniprot Description

MEP1B: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]

Protein type: EC 3.4.24.63; Membrane protein, integral; Protease

Cellular Component: membrane

Molecular Function: metalloendopeptidase activity