Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Testican-3 (SPOCK3) Standard Curve.)
Mouse Testican-3 (SPOCK3) ELISA Kit | SPOCK3 elisa kit
Mouse Testican-3 (SPOCK3) ELISA Kit
Gene Names
Spock3; AI428471; mKIAA4039; 2900045C01Rik
Reactivity
Mouse
Synonyms
Testican-3 (SPOCK3); Mouse Testican-3 (SPOCK3) ELISA Kit; SPOCK3; HSAJ1454; TES-3; testican 3; SPOCK3 elisa kit
Reactivity
Mouse
Specificity
Mouse Testican-3 (SPOCK3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPOCK3. No significant cross-reactivity or interference between Mouse SPOCK3 and analogues was observed.
Sequence Length
436
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Testican-3 (SPOCK3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Testican-3 (SPOCK3) Standard Curve.)
Related Product Information for SPOCK3 elisa kit
Description: This Mouse Testican-3 (SPOCK3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK3 in samples. An antibody specific for SPOCK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOCK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The cDNA was a splice variant of SPOCK3, which the authors called testican-3, and included the N-terminal 313-amino acid region of SPOCK3 with a 3-amino acid substitution at the C terminus; the authors designated the splice variant N-TES. N-TES contains a signal peptide, a unique domain, a follistatin-like domain, and a Ca(2+)-binding domain, but lacks a C-terminal thyroglobulin domain and 2 putative glycosaminoglycan attachment sites of SPOCK3. The full-length SPOCK3 protein contains 436 amino acids and shares 51% and 44% homology with SPOCK1 and SPOCK2, respectively. Semiquantitative RT-PCR detected expression of SPOCK3 and N-TES transcripts in normal brain; transcripts of both were downregulated in glioma tissues.
Background: The cDNA was a splice variant of SPOCK3, which the authors called testican-3, and included the N-terminal 313-amino acid region of SPOCK3 with a 3-amino acid substitution at the C terminus; the authors designated the splice variant N-TES. N-TES contains a signal peptide, a unique domain, a follistatin-like domain, and a Ca(2+)-binding domain, but lacks a C-terminal thyroglobulin domain and 2 putative glycosaminoglycan attachment sites of SPOCK3. The full-length SPOCK3 protein contains 436 amino acids and shares 51% and 44% homology with SPOCK1 and SPOCK2, respectively. Semiquantitative RT-PCR detected expression of SPOCK3 and N-TES transcripts in normal brain; transcripts of both were downregulated in glioma tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
testican-3 isoform 1
NCBI Official Synonym Full Names
sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3
NCBI Official Symbol
Spock3
NCBI Official Synonym Symbols
AI428471; mKIAA4039; 2900045C01Rik
NCBI Protein Information
testican-3
UniProt Protein Name
Testican-3
Protein Family
UniProt Gene Name
Spock3
Uniprot Description
May participate in diverse steps of neurogenesis. Inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. May interfere with tumor invasion ().
Research Articles on SPOCK3
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Product Notes
The Mouse SPOCK3 spock3 (Catalog #AAA9716846) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716846 ELISA Kit recognizes Mouse SPOCK3. It is sometimes possible for the material contained within the vial of "Testican-3 (SPOCK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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