Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Osteonectin (ON) Standard Curve.)
Human Osteonectin (ON) ELISA Kit | ON elisa kit
Human Osteonectin (ON) ELISA Kit
Gene Names
SPOCK2; testican-2
Reactivity
Human
Synonyms
Osteonectin (ON); Human Osteonectin (ON) ELISA Kit; SPOCK2; FLJ97039; testican-2; ON elisa kit
Reactivity
Human
Specificity
Human Osteonectin (ON) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOCK2. No significant cross-reactivity or interference between Human SPOCK2 and analogues was observed.
Sequence Length
424
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Osteonectin (ON) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Osteonectin (ON) Standard Curve.)
Related Product Information for ON elisa kit
Description: This Human Osteonectin (ON) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK2 in samples. An antibody specific for SPOCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. Some current research shows a correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis. Osteonectin is an acidic, secreted extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. Osteonectin also increases the production and activity of matrix metalloproteinases, a function important to invading cancer cells within bone. Additional functions of osteonectin beneficial to tumor cells include angiogenesis, proliferation and migration.
Background: Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. Some current research shows a correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis. Osteonectin is an acidic, secreted extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. Osteonectin also increases the production and activity of matrix metalloproteinases, a function important to invading cancer cells within bone. Additional functions of osteonectin beneficial to tumor cells include angiogenesis, proliferation and migration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
testican-2 isoform 2
NCBI Official Synonym Full Names
SPARC (osteonectin), cwcv and kazal like domains proteoglycan 2
NCBI Official Symbol
SPOCK2
NCBI Official Synonym Symbols
testican-2
NCBI Protein Information
testican-2
UniProt Protein Name
Testican-2
UniProt Gene Name
SPOCK2
UniProt Synonym Gene Names
KIAA0275; TICN2
UniProt Entry Name
TICN2_HUMAN
NCBI Description
This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
SPOCK2: May participate in diverse steps of neurogenesis. Binds calcium.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10pter-q25.3
Cellular Component: proteinaceous extracellular matrix
Molecular Function: glycosaminoglycan binding; extracellular matrix binding; calcium ion binding
Biological Process: extracellular matrix organization and biogenesis; synaptogenesis; regulation of cell differentiation; signal transduction; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan
Research Articles on ON
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Product Notes
The Human ON spock2 (Catalog #AAA9714002) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714002 ELISA Kit recognizes Human ON. It is sometimes possible for the material contained within the vial of "Osteonectin (ON), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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