Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Testican-2 (SPOCK2) Standard Curve.)
Mouse Testican-2 (SPOCK2) ELISA Kit | SPOCK2 elisa kit
Mouse Testican-2 (SPOCK2) ELISA Kit
Gene Names
Spock2; Gcap26; AA407235; mKIAA0275
Reactivity
Mouse
Synonyms
Testican-2 (SPOCK2); Mouse Testican-2 (SPOCK2) ELISA Kit; SPOCK2; FLJ97039; testican-2; SPOCK2 elisa kit
Reactivity
Mouse
Specificity
Mouse Testican-2 (SPOCK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPOCK2. No significant cross-reactivity or interference between Mouse SPOCK2 and analogues was observed.
Sequence Length
423
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Testican-2 (SPOCK2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Testican-2 (SPOCK2) Standard Curve.)
Related Product Information for SPOCK2 elisa kit
Description: This Mouse Testican-2 (SPOCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK2 in samples. An antibody specific for SPOCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK2 encodes a member of a novel Ca(2+)-binding proteoglycan family. The mature 402-amino acid protein has 5 domains: a follistatin-like domain, a Ca(2+)-binding domain, a thyroglobulin-like domain, and a C-terminal region with 2 putative glycosaminoglycan attachment sites. The SPOCK2 protein shares 43% overall sequence identity with SPOCK1. Northern blot analysis of adult mouse tissues revealed expression of a major 6.1-kb transcript at high levels in brain and at low levels in lung and testis. Minor bands of 4.4 and 2.6 kb were also detected.
Background: Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK2 encodes a member of a novel Ca(2+)-binding proteoglycan family. The mature 402-amino acid protein has 5 domains: a follistatin-like domain, a Ca(2+)-binding domain, a thyroglobulin-like domain, and a C-terminal region with 2 putative glycosaminoglycan attachment sites. The SPOCK2 protein shares 43% overall sequence identity with SPOCK1. Northern blot analysis of adult mouse tissues revealed expression of a major 6.1-kb transcript at high levels in brain and at low levels in lung and testis. Minor bands of 4.4 and 2.6 kb were also detected.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
testican-2
NCBI Official Synonym Full Names
sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2
NCBI Official Symbol
Spock2
NCBI Official Synonym Symbols
Gcap26; AA407235; mKIAA0275
NCBI Protein Information
testican-2
UniProt Protein Name
Testican-2
Protein Family
UniProt Gene Name
Spock2
UniProt Synonym Gene Names
Ticn2
UniProt Entry Name
TICN2_MOUSE
Uniprot Description
SPOCK2: May participate in diverse steps of neurogenesis. Binds calcium.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular region; proteinaceous extracellular matrix
Molecular Function: calcium ion binding; extracellular matrix binding; glycosaminoglycan binding
Biological Process: extracellular matrix organization and biogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; signal transduction
Research Articles on SPOCK2
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Product Notes
The Mouse SPOCK2 spock2 (Catalog #AAA9716845) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716845 ELISA Kit recognizes Mouse SPOCK2. It is sometimes possible for the material contained within the vial of "Testican-2 (SPOCK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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