Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Testican-1 (SPOCK1) Standard Curve.)
Human Testican-1 (SPOCK1) ELISA Kit | SPOCK1 elisa kit
Human Testican-1 (SPOCK1) ELISA Kit
Gene Names
SPOCK1; TIC1; SPOCK; TESTICAN
Reactivity
Human
Synonyms
Testican-1 (SPOCK1); Human Testican-1 (SPOCK1) ELISA Kit; SPOCK1; FLJ37170; SPOCK; TESTICAN; TIC1; OTTHUMP00000159433; sparc/osteonectin; cwcv and kazal-like domains proteoglycan 1; testican-1; SPOCK1 elisa kit
Reactivity
Human
Specificity
Human Testican-1 (SPOCK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOCK1. No significant cross-reactivity or interference between Human SPOCK1 and analogues was observed.
Sequence Length
439
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Testican-1 (SPOCK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Testican-1 (SPOCK1) Standard Curve.)
Related Product Information for SPOCK1 elisa kit
Description: This Human Testican-1 (SPOCK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK1 in samples. An antibody specific for SPOCK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOCK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition.The SPOCK cDNA encodes a deduced 439-amino acid protein with a predicted N-terminal signal sequence and 2 consensus glycosaminoglycan attachment sites. SPOCK, which contains osteonectin-like domains, a Kazal-like sequence, and a cys-trp-cys-val (CWCV) domain, is related to protein families that are involved in adhesion, migration, and cell proliferation.In situ hybridization showed that mouse Spock1 is located predominantly in a subpopulation of pyramidal neurons in the CA3 area of the hippocampus.
Background: TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition.The SPOCK cDNA encodes a deduced 439-amino acid protein with a predicted N-terminal signal sequence and 2 consensus glycosaminoglycan attachment sites. SPOCK, which contains osteonectin-like domains, a Kazal-like sequence, and a cys-trp-cys-val (CWCV) domain, is related to protein families that are involved in adhesion, migration, and cell proliferation.In situ hybridization showed that mouse Spock1 is located predominantly in a subpopulation of pyramidal neurons in the CA3 area of the hippocampus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
testican-1
NCBI Official Synonym Full Names
SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1
NCBI Official Symbol
SPOCK1
NCBI Official Synonym Symbols
TIC1; SPOCK; TESTICAN
NCBI Protein Information
testican-1
UniProt Protein Name
Testican-1
Protein Family
UniProt Gene Name
SPOCK1
UniProt Synonym Gene Names
SPOCK; TIC1; TICN1
NCBI Description
This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. [provided by RefSeq, Jul 2008]
Uniprot Description
SPOCK1: May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31.2
Cellular Component: cytoplasm; extracellular space; neuromuscular junction; sarcoplasm
Molecular Function: calcium ion binding; cysteine protease inhibitor activity; metalloendopeptidase inhibitor activity
Biological Process: central nervous system neuron differentiation; neurogenesis; neuron migration
Research Articles on SPOCK1
Similar Products
Product Notes
The Human SPOCK1 spock1 (Catalog #AAA9714001) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714001 ELISA Kit recognizes Human SPOCK1. It is sometimes possible for the material contained within the vial of "Testican-1 (SPOCK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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