Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble receptor activator of nuclear factor-kB ligand (sRANKL) Standard Curve.)
Mouse Soluble Receptor Activator Of Nuclear Factor-kB Ligand (sRANKL) ELISA Kit | sRANKL elisa kit
Mouse Soluble Receptor Activator Of Nuclear Factor-kB Ligand (sRANKL) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble receptor activator of nuclear factor-kB ligand (sRANKL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble receptor activator of nuclear factor-kB ligand (sRANKL) Standard Curve.)
Background: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
NCBI and Uniprot Product Information
Uniprot Description
TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; integral to membrane; plasma membrane; intracellular
Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding
Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of JNK cascade; mammary gland epithelial cell proliferation; osteoclast differentiation; lymph node development; activation of JNK activity; positive regulation of corticotropin-releasing hormone secretion; activation of NF-kappaB transcription factor; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; monocyte chemotaxis; positive regulation of MAP kinase activity; organ morphogenesis; regulation of osteoclast differentiation; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; positive regulation of T cell activation; cell differentiation; positive regulation of phosphorylation; protein homooligomerization; bone resorption
Research Articles on sRANKL
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Product Notes
The Mouse sRANKL tnfsf11 (Catalog #AAA9716728) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716728 ELISA Kit recognizes Mouse sRANKL. It is sometimes possible for the material contained within the vial of "Soluble Receptor Activator Of Nuclear Factor-kB Ligand (sRANKL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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