TRANCE/RANK L/TNFSF11 active protein

Recombinant TRANCE/RANK L/TNFSF11

Cat. Num. AAA201180

• Gene Names: Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: TRANCE/RANK L/TNFSF11; Recombinant TRANCE/RANK L/TNFSF11; CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF related activation induced cytokine; TNF-related activation-induced cytokine; TNF11_HUMAN; TNFSF 11; Tnfsf11; TRANCE; Tumor necrosis factor (ligand) superfamily member 11; Tumor necrosis factor ligand superfamily member 11; soluble form; TRANCE/RANK L/TNFSF11 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Tris-Hcl buffer(pH8.5) containing 0.1M NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 158-316aa
• Sequence: MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID
• Sequence Length: 316

• Applicable Applications for TRANCE/RANK L/TNFSF11 active protein: SDS-PAGE; Bioactivity
• Tag: Non-Tagged
• Biological Activity: Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range <= 2 ng/ml.
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Activity

(Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range )

Related Product Information for TRANCE/RANK L/TNFSF11 active protein

Tnfsf11, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Tnfsf11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. Tnfsf11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant Mouse Tnfsf11 protein was expressed in E. coli and purified by using conventional chromatography.

Product Categories/Family for TRANCE/RANK L/TNFSF11 active protein

Hormones & Cytokines

References

Lam J., et al. (2001). J. Clin. Invest. 108(7):971-9.;;Ito S., et al. (2002). J. Biol. Chem. 277(8):6631-6.

NCBI and Uniprot Product Information

• NCBI GI #: 114842415
• NCBI GeneID: 21943
• NCBI Accession #: NP_035743.2
• NCBI GenBank Nucleotide #: NP_035743.2
• UniProt Accession #: O35235
• Molecular Weight: 17.9 kDa (160aa), confirmed by MALDI-TOF
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: ODF; OPGL; RANKL; Ly109l; Trance
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_MOUSE

Uniprot Description

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; integral to membrane; plasma membrane; intracellular

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of JNK cascade; mammary gland epithelial cell proliferation; osteoclast differentiation; lymph node development; activation of JNK activity; positive regulation of corticotropin-releasing hormone secretion; activation of NF-kappaB transcription factor; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; monocyte chemotaxis; positive regulation of MAP kinase activity; organ morphogenesis; regulation of osteoclast differentiation; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; positive regulation of T cell activation; cell differentiation; positive regulation of phosphorylation; protein homooligomerization; bone resorption