Mouse tumor necrosis factor (ligand) superfamily, member 11 ELISA Kit | TNFSF11 elisa kit

Mouse soluble receptor activator of nuclear factor-kB ligand, sRANKL ELISA Kit

Cat. Num. AAA703918

• Gene Names: Tnfsf11; ODF; OPG; OPGL; RANKL; Ly109l; Trance
• Reactivity: Mouse
• Synonyms: tumor necrosis factor (ligand) superfamily; member 11; Mouse soluble receptor activator of nuclear factor-kB ligand; sRANKL ELISA Kit; CD254; ODF; OPGL; OPTB2; RANKL; TRANCE; hRANKL2; sOdf; OTTHUMP00000178585; TNF-related activation-induced cytokine; osteoclast differentiation factor; osteoprotegerin ligand; receptor activator of nucle; TNFSF11 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse TNFSF11. No significant cross-reactivity or interference between Mouse TNFSF11 and analogues was observed.
• Sequence Length: 316

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Differentiation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF11 elisa kit

Introduction: sRANKL is a part of the TNF superfamily with high similarity to other members of that protein species. Two isoforms are produced by alternate splicing, a type II membrane protein (ISOFORM 1, 317 AA, MW 35.5 kD), and a secreted molecule (ISOFORM 2, 244 AA MW 27.7kD), lacking the cytoplasmic and transmembrane domain. Although both forms are bioactive, the membrane bound protein seems to be the homeostatic form, while the production of soluble RANKL signals pathological conditions.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sRANKL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for sRANKL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sRANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sRANKL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114842415
• NCBI GeneID: 21943
• NCBI Accession #: NP_035743.2
• NCBI GenBank Nucleotide #: NM_011613.3
• UniProt Accession #: O35235; O35306; Q3TWY5; Q9JJK8; Q9JJK9; Q9R1Y0
• Molecular Weight: 35,003 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: ODF; OPG; OPGL; RANKL; Ly109l; Trance
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11; OPG ligand; osteoprotegerin ligand; osteoclast differentiation factor; receptor activator of NF-kappaB ligand; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor-related activation-induced cytokine
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_MOUSE

Uniprot Description

Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.