Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) Standard Curve.)
Human Doublesex-And MAB-3-Related Transcription Factor 2 (DMRT2) ELISA Kit | DMRT2 elisa kit
Human Doublesex-And MAB-3-Related Transcription Factor 2 (DMRT2) ELISA Kit
Reactivity
Human
Synonyms
Doublesex-And MAB-3-Related Transcription Factor 2 (DMRT2); Human Doublesex-And MAB-3-Related Transcription Factor 2 (DMRT2) ELISA Kit; DMRT2; OTTHUMP00000020964; OTTHUMP00000020966; OTTHUMP00000020967; terra-like protein; DMRT2 elisa kit
Reactivity
Human
Specificity
Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMRT2. No significant cross-reactivity or interference between Human DMRT2 and analogues was observed.
Sequence Length
226
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) Standard Curve.)
Related Product Information for DMRT2 elisa kit
Description: This Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT2 in samples. An antibody specific for DMRT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMRT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Raymond et al. (1999) sought other genes with DM domains within the minimal sex reversal region of 9p. Using a degenerate PCR strategy, they isolated a cDNA clone encoding a protein with a DM domain similar to that of DMRT1 from a human adult testis cDNA library. This cDNA, DMRT2, encodes a 226-amino acid peptide sharing 80% amino acid identity with DMRT1 in the core region of the DM domain. The 2 proteins also have an additional 29-amino acid conserved DM domain region.The authors concluded that the results provide further support for the presence of a sex-determining gene(s) on distal 9p and favor the possibility of DMRT1 and/or DMRT2 being the sex-determining gene(s).
Background: Raymond et al. (1999) sought other genes with DM domains within the minimal sex reversal region of 9p. Using a degenerate PCR strategy, they isolated a cDNA clone encoding a protein with a DM domain similar to that of DMRT1 from a human adult testis cDNA library. This cDNA, DMRT2, encodes a 226-amino acid peptide sharing 80% amino acid identity with DMRT1 in the core region of the DM domain. The 2 proteins also have an additional 29-amino acid conserved DM domain region.The authors concluded that the results provide further support for the presence of a sex-determining gene(s) on distal 9p and favor the possibility of DMRT1 and/or DMRT2 being the sex-determining gene(s).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
doublesex- and mab-3-related transcription factor 2 isoform 1
NCBI Official Synonym Full Names
doublesex and mab-3 related transcription factor 2
NCBI Official Symbol
DMRT2
NCBI Protein Information
doublesex- and mab-3-related transcription factor 2
UniProt Protein Name
Doublesex- and mab-3-related transcription factor 2
Protein Family
UniProt Gene Name
DMRT2
UniProt Synonym Gene Names
DSXL2; DSXL-2
UniProt Entry Name
DMRT2_HUMAN
NCBI Description
The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila 'doublesex' (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. [provided by RefSeq, Apr 2010]
Research Articles on DMRT2
Similar Products
Product Notes
The Human DMRT2 dmrt2 (Catalog #AAA9715674) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715674 ELISA Kit recognizes Human DMRT2. It is sometimes possible for the material contained within the vial of "Doublesex-And MAB-3-Related Transcription Factor 2 (DMRT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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