Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) Standard Curve.)
Mouse Doublesex-And MAB-3-Related Transcription Factor 1 (DMRT1) ELISA Kit | DMRT1 elisa kit
Mouse Doublesex-And MAB-3-Related Transcription Factor 1 (DMRT1) ELISA Kit
Reactivity
Mouse
Synonyms
Doublesex-And MAB-3-Related Transcription Factor 1 (DMRT1); Mouse Doublesex-And MAB-3-Related Transcription Factor 1 (DMRT1) ELISA Kit; DMRT1; DMT1; DM domain expressed in testis 1; DMRT1 elisa kit
Reactivity
Mouse
Specificity
Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRT1. No significant cross-reactivity or interference between Mouse DMRT1 and analogues was observed.
Sequence Length
374
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) Standard Curve.)
Related Product Information for DMRT1 elisa kit
Description: This Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT1 in samples. An antibody specific for DMRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes, and is found to be the key sex-determining factor in chickens. This gene exhibits a gonad-specific and sexually dimorphic expression pattern, just like the related doublesex gene in fruit flies. The protein contains a nuclear localization signal between the 2 zinc-binding sites of the DM domain, which is located in the N terminus. Immunohistochemical analysis of adult human testis revealed that DMRT1 localized to nuclei of Sertoli cells, spermatogonia, and spermatocytes.
Background: DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes, and is found to be the key sex-determining factor in chickens. This gene exhibits a gonad-specific and sexually dimorphic expression pattern, just like the related doublesex gene in fruit flies. The protein contains a nuclear localization signal between the 2 zinc-binding sites of the DM domain, which is located in the N terminus. Immunohistochemical analysis of adult human testis revealed that DMRT1 localized to nuclei of Sertoli cells, spermatogonia, and spermatocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
doublesex- and mab-3-related transcription factor 1
NCBI Official Synonym Full Names
doublesex and mab-3 related transcription factor 1
NCBI Official Symbol
Dmrt1
NCBI Protein Information
doublesex- and mab-3-related transcription factor 1
UniProt Protein Name
Doublesex- and mab-3-related transcription factor 1
Protein Family
UniProt Gene Name
Dmrt1
UniProt Entry Name
DMRT1_MOUSE
Research Articles on DMRT1
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Product Notes
The Mouse DMRT1 dmrt1 (Catalog #AAA9717849) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717849 ELISA Kit recognizes Mouse DMRT1. It is sometimes possible for the material contained within the vial of "Doublesex-And MAB-3-Related Transcription Factor 1 (DMRT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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