Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) Standard Curve.)
Human Doublesex-And MAB-3-Related Transcription Factor A2 (DMRTA2) ELISA Kit | DMRTA2 elisa kit
Human Doublesex-And MAB-3-Related Transcription Factor A2 (DMRTA2) ELISA Kit
Reactivity
Human
Synonyms
Doublesex-And MAB-3-Related Transcription Factor A2 (DMRTA2); Human Doublesex-And MAB-3-Related Transcription Factor A2 (DMRTA2) ELISA Kit; DMRTA2; OTTHUMP00000211162; DMRTA2 elisa kit
Reactivity
Human
Specificity
Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMRTA2. No significant cross-reactivity or interference between Human DMRTA2 and analogues was observed.
Sequence Length
542
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) Standard Curve.)
Related Product Information for DMRTA2 elisa kit
Description: This Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRTA2 in samples. An antibody specific for DMRTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMRTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DMRTA2 is involved in gonadal differentiation. The gene is part of five novel human DM genes that map to three well-defined regions of chromosomes 1, 9, and 19. Several studies have reported QTL in various species and strains of tilapia, regions contributing to sex determination have been identified on linkage groups 1, 3, and 23. Genes contributing to sex-specific mortality have been detected on linkage groups 2, 6, and 23. Mapping of Dax1 joined LG16 and LG21 into a single linkage group. The Amh and Dmrta2 genes were mapped to two distinct regions of LG23. The Amh gene was mapped 5 cM from UNH879 within a QTL region for sex determination and 2 cM from UNH216 within a QTL region for sex-specific mortality. Dmrta2 was mapped 4 cM from UNH848 within another QTL region for sex determination.
Background: DMRTA2 is involved in gonadal differentiation. The gene is part of five novel human DM genes that map to three well-defined regions of chromosomes 1, 9, and 19. Several studies have reported QTL in various species and strains of tilapia, regions contributing to sex determination have been identified on linkage groups 1, 3, and 23. Genes contributing to sex-specific mortality have been detected on linkage groups 2, 6, and 23. Mapping of Dax1 joined LG16 and LG21 into a single linkage group. The Amh and Dmrta2 genes were mapped to two distinct regions of LG23. The Amh gene was mapped 5 cM from UNH879 within a QTL region for sex determination and 2 cM from UNH216 within a QTL region for sex-specific mortality. Dmrta2 was mapped 4 cM from UNH848 within another QTL region for sex determination.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
doublesex- and mab-3-related transcription factor A2
NCBI Official Synonym Full Names
DMRT like family A2
NCBI Official Symbol
DMRTA2
NCBI Protein Information
doublesex- and mab-3-related transcription factor A2
UniProt Protein Name
Doublesex- and mab-3-related transcription factor A2
Protein Family
UniProt Gene Name
DMRTA2
UniProt Synonym Gene Names
DMRT5
UniProt Entry Name
DMTA2_HUMAN
Research Articles on DMRTA2
Similar Products
Product Notes
The Human DMRTA2 dmrta2 (Catalog #AAA9715672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715672 ELISA Kit recognizes Human DMRTA2. It is sometimes possible for the material contained within the vial of "Doublesex-And MAB-3-Related Transcription Factor A2 (DMRTA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
