Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) Standard Curve.)
Mouse Doublesex-And MAB-3-Related Transcription Factor 3 (DMRT3) ELISA Kit | DMRT3 elisa kit
Mouse Doublesex-And MAB-3-Related Transcription Factor 3 (DMRT3) ELISA Kit
Reactivity
Mouse
Synonyms
Doublesex-And MAB-3-Related Transcription Factor 3 (DMRT3); Mouse Doublesex-And MAB-3-Related Transcription Factor 3 (DMRT3) ELISA Kit; DMRT3; DMRTA3; MGC142144; DMRT-like family A3; testis-specific protein; DMRT3 elisa kit
Reactivity
Mouse
Specificity
Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRT3. No significant cross-reactivity or interference between Mouse DMRT3 and analogues was observed.
Sequence Length
476
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) Standard Curve.)
Related Product Information for DMRT3 elisa kit
Description: This Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT3 in samples. An antibody specific for DMRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Vertebrate DM domain genes encode a novel group of proteins related to the Drosophila doublesex and Caenorhabditis elegans mab-3 transcription factors. It is shown here that the recently identified gene, DMRT3, has a restricted embryonic expression profile that is conserved in chicken and mouse embryos. DMRT3 is expressed primarily in the forebrain, neural tube and nasal placode of both species. In the chicken, DMRT3 is also expressed in newly forming tail somites at early developmental stages and, later, in the Müllerian ducts of the urogenital system.The sequence of this gene is defined by 14 GenBank accessions from 13 cDNA clones, some from colon, PCR rescued clones (seen 2 times), thalamus (2), colon (once), colon tumor RER+ (once), lung (once), parathyroid gland (once), parathyroid tumor (once) and 4 other tissues.
Background: Vertebrate DM domain genes encode a novel group of proteins related to the Drosophila doublesex and Caenorhabditis elegans mab-3 transcription factors. It is shown here that the recently identified gene, DMRT3, has a restricted embryonic expression profile that is conserved in chicken and mouse embryos. DMRT3 is expressed primarily in the forebrain, neural tube and nasal placode of both species. In the chicken, DMRT3 is also expressed in newly forming tail somites at early developmental stages and, later, in the Müllerian ducts of the urogenital system.The sequence of this gene is defined by 14 GenBank accessions from 13 cDNA clones, some from colon, PCR rescued clones (seen 2 times), thalamus (2), colon (once), colon tumor RER+ (once), lung (once), parathyroid gland (once), parathyroid tumor (once) and 4 other tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
doublesex- and mab-3-related transcription factor 3
NCBI Official Synonym Full Names
doublesex and mab-3 related transcription factor 3
NCBI Official Symbol
Dmrt3
NCBI Protein Information
doublesex- and mab-3-related transcription factor 3
UniProt Protein Name
Doublesex- and mab-3-related transcription factor 3
Protein Family
UniProt Gene Name
Dmrt3
UniProt Entry Name
DMRT3_MOUSE
Research Articles on DMRT3
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Product Notes
The Mouse DMRT3 dmrt3 (Catalog #AAA9717848) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717848 ELISA Kit recognizes Mouse DMRT3. It is sometimes possible for the material contained within the vial of "Doublesex-And MAB-3-Related Transcription Factor 3 (DMRT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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