Testing Data
(The TFact SPI1 DNA-Binding ELISA detects active SPI1 in Hela Nuclear Extract. The Hela cells were grown 3 days in RPMI 1640 and DMEM with 10% FBS and harvested for nuclear extract. Jurkat cells were stimulated by PMA (200nM) before harvest. )
SPI1 elisa kit
SPI1 TFact DNA-Binding ELISA Kit
Testing Data
(The TFact SPI1 DNA-Binding ELISA detects active SPI1 in Hela Nuclear Extract. The Hela cells were grown 3 days in RPMI 1640 and DMEM with 10% FBS and harvested for nuclear extract. Jurkat cells were stimulated by PMA (200nM) before harvest. )
Testing Data
(The TFact SPI1 DNA-Binding ELISA detects active SPI1 in Hela Nuclear Extract. The Hela cells were grown 3 days in RPMI 1640 and DMEM with 10% FBS and harvested for nuclear extract. Jurkat cells were stimulated by PMA (200nM) before harvest. )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nuclear chromatin; nucleoplasm; nucleus
Molecular Function: DNA binding transcription factor activity; NFAT protein binding; protein binding; RNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
Biological Process: anatomical structure regression; apoptotic process involved in blood vessel morphogenesis; cellular response to ethanol; erythrocyte differentiation; granulocyte differentiation; lymphocyte differentiation; lymphoid progenitor cell differentiation; macrophage differentiation; myeloid dendritic cell differentiation; negative regulation of gene expression, epigenetic; negative regulation of histone H4 acetylation; negative regulation of MHC class II biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of erythrocyte differentiation; regulation of hematopoietic stem cell differentiation; regulation of transcription from RNA polymerase II promoter; somatic stem cell maintenance
Research Articles on SPI1
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Product Notes
The Human, Mouse, Rat SPI1 spi1 (Catalog #AAA9502165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9502165 ELISA Kit recognizes Human, Mouse, Rat SPI1. It is sometimes possible for the material contained within the vial of "SPI1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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