Rat TNF-a ELISA Kit | TNF-a elisa kit
Rat TNF-a ELISA KIT
Reactivity
Rat
Synonyms
TNF-a; Rat TNF-a ELISA KIT; TNF-a elisa kit
Reactivity
Rat
Specificity
This assay recognizes both natural and recombinant rat TNF-alpha. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Samples
Cell culture supernates, serum, and plasma
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml - 2000 pg/ml
Sensitivity
15pg/mL.
Preparation and Storage
Store at 4 degree C
Related Product Information for TNF-a elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF-alpha is added to the wells and binds to the combination of capture antibody-TNF-alpha in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TNF-alpha present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-alpha standard dilutions and TNF-alpha sample concentration determined.
Background/Introduction: The prototype ligand of theTNFsuperfamily, TNF-alpha/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis (1-4).TNF-alpha is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells (5-8). Rat TNF-alpha cDNA encodes a 235 amino acid (aa) residue type II membrane protein (9). The 156 aa residue soluble TNF-alphais released from the C-terminus of the membrane-anchored TNF-alpha by TNF-alpha-converting enzyme (TACE), a matrix metalloprotease (10).The membrane-anchored form of TNF-alpha has been shown to have lytic activity and may also have an important role in intercellular communication (11). The biologically active TNF-alpha has been shown to exist as a trimer (12, 13).TNF-alpha is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms (14). Deficiencies in either TNF-alpha or its receptors can increase susceptibility to infection by intracellular pathogens (15-16). TNF-alpha may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers (17, 18). Other potential physiological roles for TNF-alpha and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells (19-21).
Background/Introduction: The prototype ligand of theTNFsuperfamily, TNF-alpha/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis (1-4).TNF-alpha is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells (5-8). Rat TNF-alpha cDNA encodes a 235 amino acid (aa) residue type II membrane protein (9). The 156 aa residue soluble TNF-alphais released from the C-terminus of the membrane-anchored TNF-alpha by TNF-alpha-converting enzyme (TACE), a matrix metalloprotease (10).The membrane-anchored form of TNF-alpha has been shown to have lytic activity and may also have an important role in intercellular communication (11). The biologically active TNF-alpha has been shown to exist as a trimer (12, 13).TNF-alpha is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms (14). Deficiencies in either TNF-alpha or its receptors can increase susceptibility to infection by intracellular pathogens (15-16). TNF-alpha may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers (17, 18). Other potential physiological roles for TNF-alpha and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells (19-21).
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Product Notes
The Rat TNF-a (Catalog #AAA9424865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9424865 ELISA Kit recognizes Rat TNF-a. It is sometimes possible for the material contained within the vial of "TNF-a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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