IFN-gamma elisa kit

Rat IFN-gamma ELISA KIT

Cat. Num. AAA8506131

• Gene Names: IFNG; IFI; IFN-g; IFNgamma; TCRalpha; IFN-gamma
• Synonyms: IFN-gamma; Rat IFN-gamma ELISA KIT; IFN-gamma elisa kit

• Specificity: This assay recognizes both natural and recombinant mouse IFN-gamma. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
• Sequence Length: 187

• Samples: Cell Culture Supernates, Serum, And Plasma
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 7pg/mL.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFN-gamma elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-gamma is added to the wells and binds to the combination of capture antibody-IFN-gamma in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IFN-gamma present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-gamma standard dilutions and IFN-gamma sample concentration determined!!Background/Introduction: Interferon gamma (IFN-gamma) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA (1). Produced by Tlymphocytes and natural killer (NK) cells, IFN-gamma ?is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-gamma?is reported to be active only on human and non-human primate cells (5). The biochemistry and biological activities of the interferons have been extensively reviewed (2-9).Mouse IFN-gamma encodes a 155 amino acid (aa) residue precursor protein with a hydrophobic signal peptide that is cleaved to generate the 133 aa residue mature protein (10). In solution, IFN-gamma has been shown to exist as a non-covalently associated homodimer with topological similarity to IL-10. Mouse IFN-gamma shows approximately 40% aa sequence identity to human IFN-gamma and there is no cross-reactivity across species (11,12). A receptor for IFN-gamma ?has been identified and its gene localized to chromosome 6 (13,14) Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-gamma?(15-18). Functionally, IFN-gamma ?produces a variety of effects. Produced by CD8+, NK, gd,and TH1 T helper cells, IFN-gamma ?has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes (19-22) he antiprotozoal activity of IFN-gamma ?against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-gamma ?(23).The immunomodulatory effects of IFN-gamma? are extensive and diverse. In monocyte/macrophages, the activities of IFN-gamma?include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-gamma?has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 910749419
• NCBI GeneID: 101693741
• NCBI Accession #: NP_001297101.2
• NCBI GenBank Nucleotide #: NM_001310172.2
• Molecular Weight: 19,613 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFI; IFN-g; IFNgamma; TCRalpha; IFN-gamma
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma

Uniprot Description

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons ().