Rabbit anti-Human ARF5 Polyclonal Antibody | anti-ARF5 antibody

ARF5, ID (ARF5, ADP-ribosylation factor 5) (Biotin)

Cat. Num. AAA6274733

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARF5; Polyclonal Antibody; ID (ARF5; ADP-ribosylation factor 5) (Biotin); ADP-ribosylation factor 5; anti-ARF5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ARF5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the Central region of human ARF5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARF5 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARF5 antibody detected the ARF5 protein (arrow).)

Western Blot (WB)

(Western blot analysis of ARF5 (arrow) using rabbit polyclonal ARF5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARF5 gene.)

Related Product Information for anti-ARF5 antibody

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].

Product Categories/Family for anti-ARF5 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006). Austin, C., et al. Biochemistry 41(14):4669-4677(2002). Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001). Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000). Kondo, A., et al. Mol. Biol. Cell 11(4):1315-1327(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4502209
• NCBI GeneID: 381
• NCBI Accession #: NP_001653.1
• NCBI GenBank Nucleotide #: NM_001662.3
• UniProt Accession #: P84085; P26437
• Molecular Weight: 20,530 Da
• NCBI Official Full Name: ADP-ribosylation factor 5
• NCBI Official Synonym Full Names: ADP-ribosylation factor 5
• NCBI Official Symbol: ARF5
• NCBI Protein Information: ADP-ribosylation factor 5
• UniProt Protein Name: ADP-ribosylation factor 5
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF5
• UniProt Entry Name: ARF5_HUMAN

NCBI Description

This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]

Uniprot Description

ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: vesicle-mediated transport; protein transport; metabolic process; small GTPase mediated signal transduction