Rat IL-6 ELISA Kit | IL-6 elisa kit

Rat IL-6 ELISA KIT

Cat. Num. AAA9424867

• Gene Names: Il6; Il-6
• Reactivity: Rat
• Synonyms: IL-6; Rat IL-6 ELISA KIT; Rat IL-6; IL-6 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes both natural and recombinant rat IL-6. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

• Samples: cell culture supernates, serum, and plasma
• Detection Range: 125 pg/ml - 8000 pg/ml
• Sensitivity: 60pg/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

(Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for IL-6 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-6 is added to the wells and binds to the combination of capture antibody-IL-6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-6 standard dilutions and IL-6 sample concentration determined.

Background: Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis (1-5). IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction (4). The rat, mouse and human IL-6 cDNAs have been cloned (6-9). Rat IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6 (6). IL-6 is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections,trauma, autoimmune diseases, inflammations and malignancies (1-5).

NCBI and Uniprot Product Information

• NCBI GI #: 124348
• NCBI GeneID: 16193
• UniProt Accession #: P08505; Q3UCQ0; Q8BN26
• Molecular Weight: 24,384 Da
• NCBI Official Full Name: Interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: Il6
• NCBI Official Synonym Symbols: Il-6
• NCBI Protein Information: interleukin-6
• UniProt Protein Name: Interleukin-6
• UniProt Gene Name: Il6
• UniProt Synonym Gene Names: Il-6; IL-6

NCBI Description

This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5 B1|5 15.7 cM

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; interleukin-6 receptor complex

Molecular Function: cytokine activity; growth factor activity; interleukin-6 receptor binding; protein binding; receptor binding

Biological Process: cell growth; cell redox homeostasis; cytokine and chemokine mediated signaling pathway; defense response to protozoan; defense response to virus; endocrine pancreas development; glucose homeostasis; hepatic immune response; inflammatory response; muscle maintenance; myeloid cell homeostasis; negative regulation of apoptosis; negative regulation of bone resorption; negative regulation of caspase activity; negative regulation of cell proliferation; negative regulation of chemokine biosynthetic process; negative regulation of collagen biosynthetic process; negative regulation of cytokine secretion; negative regulation of gluconeogenesis; negative regulation of hormone secretion; negative regulation of membrane potential; negative regulation of muscle development; negative regulation of protein kinase activity; neurite development; neutrophil apoptosis; positive regulation of acute inflammatory response; positive regulation of apoptosis; positive regulation of B cell activation; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of interleukin-6 production; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of neuron differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein import into nucleus, translocation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translation; positive regulation of transmission of nerve impulse; positive regulation of tyrosine phosphorylation of STAT protein; regulation of apoptosis; regulation of cell proliferation; regulation of cell shape; regulation of circadian sleep/wake cycle, non-REM sleep; response to glucocorticoid stimulus; response to wounding; T cell activation