Typical Testing Data/Standard Curve (for reference only)
Rat IL-10 ELISA Kit | IL-10 elisa kit
Rat IL-10 ELISA KIT
Gene Names
IL10; CSIF; IL-10
Reactivity
Rat
Synonyms
IL-10; Rat IL-10 ELISA KIT; Rat IL-10; IL-10 elisa kit
Reactivity
Rat
Specificity
This assay recognizes both natural and recombinant rat IL-10. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross- reactivity or interference was observed.
Samples
Cell culture supernates, serum, and plasma
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml - 2000 pg/ml
Sensitivity
15pg/mL
Preparation and Storage
Store at 4 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-10 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL- 10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-10 is added to the wells and binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.
Background/Introduction: Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitor, is a pleiotropic cytokine that inhibits the production of a number of cytokines (including IL-1, GM-CSF, TNF, IL-6, IL-8, IL-12 and IFN-gamma) by activated Th1 cells, NK cells, and monocytes/macrophages(1-5). Rat IL-10 cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein (1, 2). Within the mature protein, there are two potential N-linked glycosylation sites and five cysteine residues. Mature rat IL-10 shares 85% and 74% aa sequence identity with mouse and human IL-10, respectively (1, 4). Although rat and human IL-10 are both active on mouse cells, mouse IL-10 is not active on human cells (4). Based on human and mouse studies, biologically active rat IL-10 is likely to be a nondisulfide-linked homodimer in solution (4). Although IL-10 is considered to be a strictly secreted cytokine, the existence of biologically-active, membrane-bound IL-10 has also been reported. IL-10 has been shown to inhibit macrophage cytotoxic activity and to stimulate the proliferation and differentiation of B cells, mast cells, and thymic T cells. IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM- 1 and B7 (6, 7). Finally, IL-10 has been reported to suppress the synthesis of superoxide anion (8) plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages (9). IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 (10), and on thymocytes (11) and mast cells, where it acts as a growth costimulator (12, 13).
Background/Introduction: Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitor, is a pleiotropic cytokine that inhibits the production of a number of cytokines (including IL-1, GM-CSF, TNF, IL-6, IL-8, IL-12 and IFN-gamma) by activated Th1 cells, NK cells, and monocytes/macrophages(1-5). Rat IL-10 cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein (1, 2). Within the mature protein, there are two potential N-linked glycosylation sites and five cysteine residues. Mature rat IL-10 shares 85% and 74% aa sequence identity with mouse and human IL-10, respectively (1, 4). Although rat and human IL-10 are both active on mouse cells, mouse IL-10 is not active on human cells (4). Based on human and mouse studies, biologically active rat IL-10 is likely to be a nondisulfide-linked homodimer in solution (4). Although IL-10 is considered to be a strictly secreted cytokine, the existence of biologically-active, membrane-bound IL-10 has also been reported. IL-10 has been shown to inhibit macrophage cytotoxic activity and to stimulate the proliferation and differentiation of B cells, mast cells, and thymic T cells. IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM- 1 and B7 (6, 7). Finally, IL-10 has been reported to suppress the synthesis of superoxide anion (8) plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages (9). IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 (10), and on thymocytes (11) and mast cells, where it acts as a growth costimulator (12, 13).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,934 Da
NCBI Official Full Name
IL-10, partial
NCBI Official Symbol
IL10
NCBI Official Synonym Symbols
CSIF; IL-10
NCBI Protein Information
interleukin-10
UniProt Protein Name
Interleukin-10
UniProt Gene Name
IL10
UniProt Synonym Gene Names
IL-10; CSIF
Uniprot Description
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Research Articles on IL-10
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Product Notes
The Rat IL-10 il10 (Catalog #AAA9424868) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9424868 ELISA Kit recognizes Rat IL-10. It is sometimes possible for the material contained within the vial of "IL-10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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