Mouse wingless-type MMTV integration site family, member 5A ELISA Kit | WNT5A elisa kit

Mouse Protein Wnt-5a, WNT5A ELISA Kit

Cat. Num. AAA932852

• Gene Names: Wnt5a; Wnt-5a; 8030457G12Rik
• Reactivity: Mouse
• Synonyms: wingless-type MMTV integration site family; member 5A; Mouse Protein Wnt-5a; WNT5A ELISA Kit; Mouse Protein Wnt-5a (WNT5A) ELISA kit; hWNT5A; WNT-5A protein; WNT5A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse WNT5A. No significant cross-reactivity or interference between mouse WNT5A and analogues was observed.
• Sequence Length: 360

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml -200 pg/ml.
• Sensitivity: The minimum detectable dose of mouse WNT5A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT5A elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

Ah Receptor Activation by Dioxin Disrupts Activin, BMP, and WNT Signals During the Early Differentiation of Mouse Embryonic Stem Cells and Inhibits Cardiomyocyte Functions. Qin Wang, Hisaka Kurita, Vinicius Carreira, Chia-I Ko, Yunxia Fan, Xiang Zhang, Jacek Biesiada, Mario Medvedovic and Alvaro Puga (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 371940979
• NCBI GeneID: 22418
• NCBI Accession #: NP_001243153.1
• NCBI GenBank Nucleotide #: NM_001256224.1
• UniProt Accession #: P22725; Q8BM17; Q8BMF9; Q8VCV6
• Molecular Weight: 42,309 Da
• NCBI Official Full Name: protein Wnt-5a isoform 2
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 5A
• NCBI Official Symbol: Wnt5a
• NCBI Official Synonym Symbols: Wnt-5a; 8030457G12Rik
• NCBI Protein Information: protein Wnt-5a; wingless-related MMTV integration site 5A
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: Wnt5a
• UniProt Synonym Gene Names: Wnt-5a
• UniProt Entry Name: WNT5A_MOUSE

Uniprot Description

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular

Molecular Function: protein domain specific binding; receptor tyrosine kinase-like orphan receptor binding; protein binding; frizzled binding; cytokine activity; transcription factor activity; frizzled-2 binding; receptor binding

Biological Process: neural tube development; positive regulation of JNK activity; activation of MAPK activity; multicellular organismal development; embryonic skeletal development; positive regulation of transcription, DNA-dependent; negative chemotaxis; positive regulation of endocytosis; Wnt receptor signaling pathway through beta-catenin; positive regulation of interleukin-1 beta secretion; negative regulation of synaptogenesis; uterus development; protein amino acid phosphorylation; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; anterior/posterior pattern formation; positive regulation of fibroblast proliferation; regulation of protein localization; cell-cell signaling; convergent extension; determination of anterior/posterior axis, embryo; positive regulation of mesenchymal cell proliferation; JNK cascade; embryonic limb morphogenesis; positive regulation of macrophage activation; positive regulation of cytokine production; somitogenesis; inner ear morphogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; positive regulation of interleukin-6 production; vagina development; digestive tract morphogenesis; negative regulation of fat cell differentiation; genitalia development; organ morphogenesis; positive regulation of angiogenesis; Wnt receptor signaling pathway, calcium modulating pathway; positive regulation of protein catabolic process; midgut development; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; determination of left/right symmetry; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; axon guidance; wound healing; negative regulation of fibroblast growth factor receptor signaling pathway; hypophysis morphogenesis; positive regulation of cytokine secretion during immune response; positive regulation of JNK cascade; palate development; negative regulation of axon extension involved in axon guidance; signal transduction; activation of JNK activity; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; morphogenesis of an epithelium; establishment of planar polarity; heart looping; cell differentiation; negative regulation of epithelial cell proliferation; cervix development; cell migration; Wnt receptor signaling pathway; positive regulation of meiosis; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; positive regulation of peptidyl-serine phosphorylation; limb morphogenesis; positive regulation of ossification; positive regulation of cGMP metabolic process; development of primary male sexual characteristics; convergent extension involved in organogenesis; embryonic development; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; cartilage development; ameboidal cell migration; neural tube closure; hindgut morphogenesis; positive regulation of protein amino acid phosphorylation; neurite morphogenesis; positive regulation of inflammatory response; lung development