SUPT5H blocking peptide

SUPT5H Antibody (Center) Blocking Peptide

• Gene Names: SUPT5H; SPT5; SPT5H; Tat-CT1
• Synonyms: SUPT5H; SUPT5H Antibody (Center) Blocking Peptide; Transcription elongation factor SPT5; hSPT5; DRB sensitivity-inducing factor 160 kDa subunit; DSIF p160; DRB sensitivity-inducing factor large subunit; DSIF large subunit; Tat-cotransactivator 1 protein; Tat-CT1 protein; SPT5; SPT5H; SUPT5H blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1087

• Cellular Location: Nucleus.
• Tissue Location: Ubiquitously expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SUPT5H blocking peptide

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

NCBI and Uniprot Product Information

• NCBI GI #: 74735318
• NCBI GeneID: 6829
• UniProt Accession #: O00267; O43279; Q59G52; Q99639
• Molecular Weight: 120,499 Da
• NCBI Official Full Name: Transcription elongation factor SPT5
• NCBI Official Synonym Full Names: SPT5 homolog, DSIF elongation factor subunit
• NCBI Official Symbol: SUPT5H
• NCBI Official Synonym Symbols: SPT5; SPT5H; Tat-CT1
• NCBI Protein Information: transcription elongation factor SPT5
• UniProt Protein Name: Transcription elongation factor SPT5
• UniProt Gene Name: SUPT5H
• UniProt Synonym Gene Names: SPT5; SPT5H; hSPT5; DSIF p160; DSIF large subunit; Tat-CT1 protein
• UniProt Entry Name: SPT5H_HUMAN

Uniprot Description

SPT5: a transcription elongation factor. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Two alternatively spliced human isoforms have been described.

Protein type: RNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: enzyme binding; protein binding; protein heterodimerization activity

Biological Process: mRNA capping; negative regulation of RNA elongation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of macroautophagy; positive regulation of RNA elongation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral transcription; response to organic substance; RNA elongation; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter