Rabbit SUPT5H Polyclonal Antibody | anti-SUPT5H antibody

SUPT5H Antibody (Center)

Cat. Num. AAA9210198

• Gene Names: SUPT5H; SPT5; SPT5H; Tat-CT1
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SUPT5H; Polyclonal Antibody; SUPT5H Antibody (Center); Transcription elongation factor SPT5; hSPT5; DRB sensitivity-inducing factor 160 kDa subunit; DSIF p160; DRB sensitivity-inducing factor large subunit; DSIF large subunit; Tat-cotransactivator 1 protein; Tat-CT1 protein; SPT5; SPT5H; anti-SUPT5H antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the Central region of human SUPT5H.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.3 (varies by lot)
• Sequence Positions: 395-424
• Sequence Length: 1087

• Applicable Applications for anti-SUPT5H antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(SUPT5H Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SUPT5H antibody detected the SUPT5H protein (arrow).)

Related Product Information for anti-SUPT5H antibody

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

References

Wenzel, S., et al. Biochem. J. 425(2):373-380(2010)
Chen, Y., et al. Genes Dev. 23(23):2765-2777(2009)
Komori, T., et al. Genes Cells 14(3):343-354(2009)
Chen, H., et al. PLoS ONE 4 (9), E6918 (2009) :
Amir-Zilberstein, L., et al. J. Biol. Chem. 283(3):1317-1323(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 161169023
• NCBI GeneID: 6829
• NCBI Accession #: NP_001104490.1; NP_001124296.1; NP_001124297.1; NP_003160.2
• NCBI GenBank Nucleotide #: NM_001111020.2
• UniProt Accession #: O00267; O43279; Q59G52; Q99639
• Molecular Weight: 121000
• NCBI Official Full Name: transcription elongation factor SPT5 isoform a
• NCBI Official Synonym Full Names: suppressor of Ty 5 homolog (S. cerevisiae)
• NCBI Official Symbol: SUPT5H
• NCBI Official Synonym Symbols: SPT5; SPT5H; Tat-CT1
• NCBI Protein Information: transcription elongation factor SPT5
• UniProt Protein Name: Transcription elongation factor SPT5
• UniProt Gene Name: SUPT5H
• UniProt Synonym Gene Names: SPT5; SPT5H; hSPT5; DSIF p160; DSIF large subunit; Tat-CT1 protein
• UniProt Entry Name: SPT5H_HUMAN

Uniprot Description

SPT5: a transcription elongation factor. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Two alternatively spliced human isoforms have been described.

Protein type: Transcription, coactivator/corepressor; RNA-binding

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; protein heterodimerization activity; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of RNA elongation; negative regulation of RNA elongation; negative regulation of transcription from RNA polymerase II promoter; cell cycle; response to organic substance; chromatin remodeling; mRNA capping; RNA elongation; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macroautophagy