Rabbit anti-Human SUPT5H Polyclonal Antibody | anti-SUPT5H antibody

SUPT5H, ID (SUPT5H, SPT5, SPT5H, Transcription elongation factor SPT5, DRB sensitivity-inducing factor 160kD subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein) (APC)

Cat. Num. AAA6352633

• Gene Names: SUPT5H; SPT5; SPT5H; Tat-CT1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SUPT5H; Polyclonal Antibody; ID (SUPT5H; SPT5; SPT5H; Transcription elongation factor SPT5; DRB sensitivity-inducing factor 160kD subunit; DRB sensitivity-inducing factor large subunit; Tat-cotransactivator 1 protein) (APC); Tat-cotransactivator 1 protein; anti-SUPT5H antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SUPT5H antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-424 amino acids from the Central region of human SUPT5H.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SUPT5H Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SUPT5H antibody detected the SUPT5H protein (arrow).)

Related Product Information for anti-SUPT5H antibody

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Product Categories/Family for anti-SUPT5H antibody

Antibodies; Transcription Factors

References

Wenzel, S., et al. Biochem. J. 425(2):373-380(2010). Chen, Y., et al. Genes Dev. 23(23):2765-2777(2009). Komori, T., et al. Genes Cells 14(3):343-354(2009). Chen, H., et al. PLoS ONE 4 (9), E6918 (2009): Amir-Zilberstein, L., et al. J. Biol. Chem. 283(3):1317-1323(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 161169023
• NCBI GeneID: 6829
• NCBI Accession #: NP_001104490.1; NP_001124297.1; NP_001124296.1
• NCBI GenBank Nucleotide #: NM_001111020.2
• UniProt Accession #: O00267; O43279; Q59G52; Q99639
• Molecular Weight: 121,000 Da
• NCBI Official Full Name: transcription elongation factor SPT5 isoform a
• NCBI Official Synonym Full Names: suppressor of Ty 5 homolog (S. cerevisiae)
• NCBI Official Symbol: SUPT5H
• NCBI Official Synonym Symbols: SPT5; SPT5H; Tat-CT1
• NCBI Protein Information: transcription elongation factor SPT5; hSPT5; DSIF p160; DSIF large subunit; Tat-cotransactivator 1 protein; DRB sensitivity-inducing factor large subunit; DRB sensitivity-inducing factor 160 kDa subunit
• UniProt Protein Name: Transcription elongation factor SPT5
• UniProt Gene Name: SUPT5H
• UniProt Synonym Gene Names: SPT5; SPT5H
• UniProt Entry Name: SPT5H_HUMAN