Rabbit anti-Human HIPK1 Polyclonal Antibody | anti-HIPK1 antibody

HIPK1, CT (HIPK1, KIAA0630, MYAK, NBAK2, Homeodomain-interacting protein kinase 1, Nuclear body-associated kinase 2) (AP)

Cat. Num. AAA6306560

• Gene Names: HIPK1; Myak; Nbak2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIPK1; Polyclonal Antibody; CT (HIPK1; KIAA0630; MYAK; NBAK2; Homeodomain-interacting protein kinase 1; Nuclear body-associated kinase 2) (AP); Nuclear body-associated kinase 2; anti-HIPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1210

• Applicable Applications for anti-HIPK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HIPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 892-922 amino acids from the C-terminal region of human HIPK1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HIPK1 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. HIPK1 Antibody (C-term) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HIPK1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIPK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HIPK1 antibody

The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms.

Product Categories/Family for anti-HIPK1 antibody

Antibodies; Protein Kinases

References

Matre, V., et al. Biochem. Biophys. Res. Commun. 388(1):150-154(2009). Li, X., et al. J. Biol. Chem. 280(15):15061-15070(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Song, J.J., et al. J. Biol. Chem. 278(47):47245-47252(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 38201640
• NCBI GeneID: 204851
• NCBI Accession #: NP_938009.1; NP_938010.1
• NCBI GenBank Nucleotide #: NP_938009.1
• UniProt Accession #: Q86Z02
• NCBI Official Full Name: homeodomain-interacting protein kinase 1 isoform 1
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 1
• NCBI Official Symbol: HIPK1
• NCBI Official Synonym Symbols: Myak; Nbak2
• NCBI Protein Information: homeodomain-interacting protein kinase 1
• UniProt Protein Name: Homeodomain-interacting protein kinase 1
• UniProt Gene Name: HIPK1
• UniProt Synonym Gene Names: KIAA0630; MYAK; NBAK2
• UniProt Entry Name: HIPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

HIPK1: homeodomain interacting protein kinase 1 is a CMGC kinase of the DYRK family. May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Expression elevated in many breast cancer cell lines. Null mice are resistant to carcinogens. Binds the p53 tumor suppressor. 4 alternatively spliced isoforms of the human protein have been described.

Protein type: Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; DYRK family; HIPK subfamily

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: PML body; cytoplasm; nuclear speck; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: neuron differentiation; anterior/posterior pattern formation; positive regulation of angiogenesis; smoothened signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; eye development; embryonic retina morphogenesis in camera-type eye; positive regulation of cell proliferation; embryonic camera-type eye morphogenesis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein amino acid phosphorylation