Related Product Information for anti-Rps6kb1 antibody
Rps6kb1 acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-136' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-136'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates POLDIP3 (By similarity).
Product Categories/Family for anti-Rps6kb1 antibody
References
Lai, K.P., et al. EMBO J. 29(17):2994-3006(2010)
Michels, A.A., et al. Mol. Cell. Biol. 30(15):3749-3757(2010)
Witkowski, S., et al. FEBS Lett. 584(13):2891-2895(2010)
Carnevalli, L.S., et al. Dev. Cell 18(5):763-774(2010)
Hamilton, D.L., et al. BMC Cell Biol. 11, 37 (2010) :
Uniprot Description
p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.
Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Translation; AGC group; RSK family; p70 subfamily
Cellular Component: nucleoplasm; mitochondrial outer membrane; neuron projection; cell surface; membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; synapse; intracellular; cell junction; nucleus
Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ribosomal protein S6 kinase activity; peptide binding; ATP binding; protein kinase activity
Biological Process: TOR signaling pathway; behavioral fear response; positive regulation of translation; apoptosis; positive regulation of smooth muscle cell proliferation; negative regulation of insulin receptor signaling pathway; cell cycle; signal transduction; positive regulation of mitotic cell cycle; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; germ cell development; response to insulin stimulus; positive regulation of translational initiation; regulation of translation; cellular response to insulin stimulus; protein kinase B signaling cascade; regulation of glucose import; positive regulation of skeletal muscle growth; phosphorylation; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Product Notes
The Rps6kb1 rps6kb1 (Catalog #
AAA9204564) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 481-509. The Mouse Rps6kb1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Rps6kb1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Rps6kb1 rps6kb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"Rps6kb1, Polyclonal Antibody" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
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to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
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